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| Status |
Public on Nov 20, 2015 |
| Title |
ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we demonstrated that deletion of the activating transcription factor 4 (ATF4) resulted in severely impaired HSC expansion in the fetal liver at E12.5 and E15.5. In contrast, generation of the first HSC population in the aorta-gonad-mesonephros region at E11.5 was not significantly affected. Furthermore, the HSC-supporting ability of both endothelial and stromal cells in fetal liver was significantly compromised in the absence of ATF4. Gene profiling using RNA-seq revealed down-regulated expression of a panel of cytokines in ATF4-/- stromal cells, including angiopoietin-like protein 3 (Angptl3) and vascular endothelial growth factor-A (VEGFA).
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| Overall design |
To investigate the molecular pathways of ATF4 in the stromal cells and LSK cells in the fetal liver.
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| Contributor(s) |
Cheng T, Liu B, Zhao Y, Zhou J, Liu D, Dong F, Cheng H, Wang W |
| Citation(s) |
26384355 |
| |
| Submission date |
Feb 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
dan liu |
| E-mail(s) |
liudan@big.ac.cn
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| Organization name |
Beijing Institute of Genomics, Chinese Academy of Sciences
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| Street address |
NO.1 Beichen West Road, Chaoyang District, Beijing
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE66382 |
ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment |
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| Relations |
| BioProject |
PRJNA276686 |
| SRA |
SRP055670 |
