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Series GSE66268 Query DataSets for GSE66268
Status Public on Apr 12, 2015
Title Regulation of T cell cytokine expression by Tet2-mediated DNA demethylation [MeDIP-Seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary T cell function is regulated by epigenetic mechanisms. 5-methylcytosine (5mC) conversion to 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation (Tet) proteins was identified to mediate DNA demethylation. Here, we characterize the genome-wide distribution of 5hmC in T cells using DNA immunoprecipitation coupled with high-throughput DNA sequencing. 5hmC marks signature genes associated with effector cell differentiation in the putative regulatory elements. Moreover, Tet2 protein is recruited to 5hmC-containing regions, dependent on lineage-specific transcription factors. Deletion of the Tet2 gene in T cells decreased their cytokine expression, associated with reduced p300 recruitment. In vivo, Tet2 plays a critical role in the expression of cytokine genes. Collectively, our findings for the first time demonstrate a key role of Tet-mediated active DNA demethylation in T cells.
Overall design Examination of 5mC and 5hmC patterns in helper T cell subsets including naïve, Th1, Th2, Th17, and iTreg cells.
Contributor(s) Ichiyama K, Wei L, Dong C
Citation(s) 25862091
Submission date Feb 24, 2015
Last update date May 15, 2019
Contact name Raymond Doty
Organization name University of Washington
Department Division of Hematology
Street address Box 357710
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (15)
GSM1618370 Input
GSM1618371 5mC.Naive
GSM1618372 5mC.Th1
This SubSeries is part of SuperSeries:
GSE66945 Regulation of T cell cytokine expression by Tet2-mediated DNA demethylation
BioProject PRJNA276287
SRA SRP055480

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66268_RAW.tar 13.0 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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