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Series GSE66174 Query DataSets for GSE66174
Status Public on Feb 12, 2017
Title Differentiation of mammary epithelial cells with/without exposure to RANKL.
Organism Mus musculus
Experiment type Expression profiling by array
Summary In order to investigate the mechanisms underlying defective alveologenesis caused by RANK over-expression in mice, global gene expression profiles from primary acinar cultures of mammary epithelial cells were analyzed.
 
Overall design Mammary epithelial cells from gestation day 16.5 WT and MMTV-RANK females were obtained. Cultures were established in the presence of prolactin, which induces differentiation into milk secreting acini, and with or without RANKL (Receptor Activator of NFkB Ligand) to precisely determine the role of RANK signaling at midgestation. Global gene expression profiling at 8 hours (h), 24 h and 72 h from WT, and MMTV-RANK 3D acinar cultures was obtained.

Please note that experiments were performed technically as dual channel (e.g., Cy3 and Cy5-labeled samples hybridized to the same array; total 72 hybridizations) but the results were processed as though they were single channel. Rosetta Resolver ratio split algorithm was used to parse individual Cy3 (Ch1) or Cy5 (Ch2) intensity values and then to combine 4 replicate values for each sample into a single intensity value. Therefore, the normalized_combined_data.txt (available on the Series records) contains 36 data columns [i.e. (72array X 2 channel)/4 replicates; 2 genotype (WT or TG) X 3 replicates X 3 timepoints (8 ,24 ,72hr) X 2 treatments (w/&w/o RANKL)].
 
Contributor(s) Gonzalez-Suarez E, Cordero A, Deshpande C
Citation(s) 27241552
Submission date Feb 20, 2015
Last update date Feb 12, 2017
Contact name Eva González-Suárez
E-mail(s) egsuarez@idibell.cat
Organization name Institut d'Investigació Biomèdica de Bellvitge
Department Epigenetics and Cancer Biology
Lab Transformation and Metastasis
Street address Hospital Duran i Reynals - 3a planta -Gran Via de l'Hospitalet, 199
City Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platforms (1)
GPL2872 Agilent-012694 Whole Mouse Genome G4122A (Feature Number version)
Samples (72)
GSM1616206 Acini_MECs_WT 670-8h 1_WT RL 670-8h 2
GSM1616207 Acini_MECs_WT RL 670-8h 2_WT 670-8h 1
GSM1616208 Acini_MECs_WT 670-24h 17_WT RL 670-24h 18
Relations
BioProject PRJNA276015

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66174_RAW.tar 743.6 Mb (http)(custom) TAR (of TXT)
GSE66174_normalized_combined_data.txt.gz 4.2 Mb (ftp)(http) TXT
Processed data are available on Series record

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