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| Status |
Public on Sep 24, 2015 |
| Title |
Neonatal naïve CD8+ T cells have effector-like gene expression that prevents memory cell formation [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Neonates are intrinsically defective at creating memory CD8+ T cells in response to infection with intracellular pathogens. Here we investigated differential of small RNAs, transcription factors, and chemokine receptors regulation in neonates as compared to adults before and during infection. We found that prior to infection, naïve cells have a different expression profile for many microRNAs, and gene targets of these microRNAs show widespread expression differences. These targets and other changes in gene expression in naïve cells result in neonatal cells that get activated more easily, express chemokine receptors that home to sites of infection, and are less protected from apoptosis during contraction. As a result, changes in neonatal naïve cells drive effector cell terminal differentiation at the expense of creating long-lived memory cells.
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| Overall design |
total RNAs were sequenced from adult and neonatal CD8+ T cells before and during infection
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| Contributor(s) |
Wissink E, Grimson A |
| Citation(s) |
26416483 |
| |
| Submission date |
Feb 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Grimson |
| Organization name |
Cornell University
|
| Department |
Molecular Biology & Genetics
|
| Street address |
445 Biotechnology Building
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE65923 |
Neonatal naïve CD8+ T cells have effector-like gene expression that prevents memory cell formation |
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| Relations |
| BioProject |
PRJNA275416 |
| SRA |
SRP055023 |
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