NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE65914 Query DataSets for GSE65914
Status Public on Jun 01, 2015
Title Th1/Th17 Immune Response in Rosacea
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Rosacea is a common chronic inflammatory skin disease of unknown etiology. Our knowledge about an involvement of the adaptive immune system is very limited. We performed detailed transcriptome analysis, qRT-PCR, and quantitative immunohistochemistry on facial biopsies of rosacea patients, classified according to their clinical subtype. As controls, we used samples from healthy controls. Our study shows significant activation of the immune system in all subtypes of rosacea, characterizing erythematotelangiectatic rosacea (ETR) already as a disease with significant influx of proinflammatory cells. The T cell response is dominated by Th1/Th17-polarized immune cells, as demonstrated by significant upregulation of IFNγ or IL-17, for example. Chemokine expression patterns support a Th1/Th17 polarization profile of the T cell response. Macrophages and mast cells are increased in all three subtypes of rosacea, while neutrophils reach a maximum in papulopustular rosacea. Our studies also provide evidence for activation of plasma cells with significant antibody production already in ETR, followed by a crescendo pattern towards phymatous rosacea. In sum, Th1/Th17 polarized inflammation and macrophage infiltration is an underestimated hallmark in all subtypes of rosacea. Therapies directly targeting the Th1/Th17 pathway are promising candidates in the future treatment of this skin disease.
 
Overall design Total of 58 chips. 19 patients and 10 healthy volunteers
 
Contributor(s) Voegel JJ, Steinhoff M
Citation(s) 25848978
Submission date Feb 13, 2015
Last update date Mar 25, 2019
Contact name Isabelle Carlavan
E-mail(s) isabelle.carlavan@galderma.com
Organization name Galderma R&D
Department Research
Street address 2400 route des colles
City Sophia Antipolis
ZIP/Postal code 06902
Country France
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (58)
GSM1611035 Healthy volunteer [GRDS0027_HV1_1]
GSM1611036 Healthy volunteer [GRDS0027_HV1_2]
GSM1611037 Healthy volunteer [GRDS0027_HV2_1]
Relations
BioProject PRJNA275409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65914_RAW.tar 274.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap