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Series GSE65894 Query DataSets for GSE65894
Status Public on Jul 28, 2015
Title Transcriptome comparison between Bacillus cereus ATCC 14579 wildtype (WT), RpoN (Sigma 54) deletion mutant and the complemented strain.
Organism Bacillus cereus ATCC 14579
Experiment type Expression profiling by array
Summary RpoN was shown to have a pleiotropic role in different microorganisms. This study was performed to elucidate role in B. cereus.
In this study we compared the transcriptomic profiles of the WT, rpoN mutant and the complemented strain in aerated and static growth conditions.
 
Overall design Matrix is compatible with the Agilent-017343 array design. Aerated cultures were sampled at two time points, upon reaching OD (600 nm) values of 0.2 (t1) and 1.0 (t2), which corresponded to mid-exponential and transition growth phases, respectively. Statically grown cultures were sampled at OD=0.2 (600 nm) corresponding to exponential growth (exp/static). Two independent biological replicates were hybridized on the arrays with either 2 (WT) or 3 (Δrpon and ΔrpoN-comp) technical replicates for each biological replicate.
 
Contributor(s) Hayrapetyan H, Boekhorst J, Tempelaars M, Nierop Groot M, Abee T
Citation(s) 26241851
Submission date Feb 12, 2015
Last update date Oct 10, 2019
Contact name Jos Boekhorst
E-mail(s) Jos.Boekhorst@nizo.com
Organization name NIZO food research
Street address Kernhemseweg 2
City Ede
ZIP/Postal code 6718 ZB
Country Netherlands
 
Platforms (1)
GPL19768 Bacillus cereus ATCC 14579 - design 3
Samples (24)
GSM1608449 SH t1 rpoN biol1 over SH t1 WT biol1
GSM1608450 SH t1 rpoN biol1 over SH t1 rpoN_compl biol2
GSM1608451 SH t1 rpoN biol1 over SH t1 rpoN biol2
Relations
BioProject PRJNA275326

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65894_RAW.tar 76.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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