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| Status |
Public on Jul 03, 2020 |
| Title |
Low-grade glioma microglia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To examine the effect of Nf1 inactivation in astrocytes on normal microglia in the optic nerve, microglia from Nf1 flox/flox (FF) and Nf1 flox/flox; GFAP-Cre (FFC) were flow sorted. High throughput RNA-seq was employed to compare the expression profiles, and upregulated genes in microglia supportive to Nf1 deficient astrocytes were verified.
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| |
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| Overall design |
Microglia from mice lacking Nf1 in astrocytes were flow sorted. Upregulated genes were verified and further examined.
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| Contributor(s) |
Pong WW, Walker J, Wylie T, Magrini V, Griffith M, Griffith OL, Mardis ER, Gutmann DH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 11, 2015 |
| Last update date |
Jul 03, 2020 |
| Contact name |
Winnie W Pong |
| Organization name |
Washington University School of Medicine
|
| Department |
Department of Neurology
|
| Lab |
David H Gutmann Laboratory
|
| Street address |
660 S. Euclid Avenue
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA275388 |
| SRA |
SRP055010 |
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