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Series GSE65865 Query DataSets for GSE65865
Status Public on Jul 03, 2020
Title Low-grade glioma microglia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To examine the effect of Nf1 inactivation in astrocytes on normal microglia in the optic nerve, microglia from Nf1 flox/flox (FF) and Nf1 flox/flox; GFAP-Cre (FFC) were flow sorted. High throughput RNA-seq was employed to compare the expression profiles, and upregulated genes in microglia supportive to Nf1 deficient astrocytes were verified.
Overall design Microglia from mice lacking Nf1 in astrocytes were flow sorted. Upregulated genes were verified and further examined.
Contributor(s) Pong WW, Walker J, Wylie T, Magrini V, Griffith M, Griffith OL, Mardis ER, Gutmann DH
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Submission date Feb 11, 2015
Last update date Jul 03, 2020
Contact name Winnie W Pong
Organization name Washington University School of Medicine
Department Department of Neurology
Lab David H Gutmann Laboratory
Street address 660 S. Euclid Avenue
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM1608021 11Nov03_FF
GSM1608022 11Nov08_FF
GSM1608023 11Nov15_FF
BioProject PRJNA275388
SRA SRP055010

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Supplementary file Size Download File type/resource
GSE65865_FFC-FF_gene_fpkm.tsv.gz 1.5 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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