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| Status |
Public on Mar 31, 2015 |
| Title |
Transcriptome response of Salmonella enterica subsp. enterica serovar Typhimurium N-15 and Bifidobacterium thermophilum RBL67 in co-culture |
| Organisms |
Bifidobacterium thermophilum; Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bifidobacterium thermophilum RBL67 (RBL67), a human fecal isolate and promising probiotic candidate, showed antagonistic and protective effects against Salmonella and Listeria in vitro. However, the underlying mechanisms fostering these health-related effects remain unknown. Therefor the transcriptome response of RBL67 and Salmonella enterica subsp. enterica serovar Typhimurium N-15 (N-15) in co-culture compared to the response in their respective mono-cultures. RNA was extracted from culture samples taken after 4 (N-15) or 5 h (RBL67) and RNAseq was performed on an Illumina HiSeq 2000 sequencer. Three biological replciates were performed resulting in 12 data sets: 3 RBL67 mono culture, 3 N15 mono-culture, 3 RBL67 co-culture, 3 N15 co-culture. Our study provided first insights into probiotic-pathogen interaction on transcriptional level and suggests a mechanism for how probiotic organisms can protect the host from infections.
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| Overall design |
RNA was extracted from culture samples taken after 4 (N-15) or 5 h (RBL67) and RNAseq was performed on an Illumina HiSeq 2000 sequencer. Three biological replciates were performed resulting in 12 data sets: 3 RBL67 mono culture, 3 N15 mono-culture, 3 RBL67 co-culture, 3 N15 co-culture.
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| Contributor(s) |
Tanner SA, Chassard C, Lacroix C, Rigozzi E, Stevens MJ |
| Citation(s) |
26988691 |
| |
| Submission date |
Feb 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Marc Stevens |
| E-mail(s) |
marc.stevens@uzh.ch
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| Organization name |
University of Zurich
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| Department |
Vetsuisse
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| Lab |
Institute for Food Safety and Hygiene
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| Street address |
Winterthurerstrasse 272
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| City |
Zürich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL17070 |
Illumina HiSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium) |
| GPL19749 |
Illumina HiSeq 2000 (Bifidobacterium thermophilum) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA274782 |
| SRA |
SRP053305 |
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