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| Status |
Public on May 19, 2015 |
| Title |
Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Promiscuous gene expression (pGE) of numerous self-antigens in thymic epithelial cells (TEC) enables the elimination of self-reactive T cells. The autoimmune regulator (Aire) is the only known molecular determinant driving pGE in the thymus but the existence of Aire-independent mechanisms has been inferred. Here, we analyzed the poly(A)+ transcriptome of TEC populations by RNA-sequencing (RNA-seq) in order to reveal differential features of Aire-induced vs. –independent pGE. We report an unanticipated effect of Aire deletion on the proliferation and differentiation of cortical TEC. Moreover, the RNA-seq data reveal the breath of Aire-induced and –independent pGE in medullary TEC (mTEC) subsets and the extent of thymic peripheral tissue representation. The results suggest that Aire-induced promiscuously expressed transcripts affect several functions with far reaching biological consequences in mTEC. High-throughput characterization of TEC transcriptomes will enable progress in understanding TEC biology and the establishment of self-tolerance.
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| Overall design |
The mRNA profiles of cTEC, mTEClo and mTEChi from 6-8 week-old wild type (WT) and Aire-/- (KO) mice were generated by RNA-sequencing using Illumina HiSeq2000.
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| Contributor(s) |
St-Pierre C, Perreault C |
| Citation(s) |
26034170, 32345368 |
| |
| Submission date |
Feb 04, 2015 |
| Last update date |
May 12, 2020 |
| Contact name |
Charles St-Pierre |
| E-mail(s) |
charles.stpierre78@gmail.com
|
| Organization name |
Montreal University
|
| Department |
Institute for Research in Immunology and Cancer
|
| Lab |
Immunobiology - Claude Perreault
|
| Street address |
2950, Chemin de Polytechnique
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1J4 |
| Country |
Canada |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA274486 |
| SRA |
SRP053177 |
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