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| Status |
Public on Jul 01, 2015 |
| Title |
Pre-ovulatory follicle size modulates the periovulatory endocrine environment and endometrial tissue phenotype in cattle |
| Organism |
Bos indicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpouse: Aims of this study were (1) to characterize the endometrial transcriptome, (2) to identify functional pathways, and (3) to molecularly characterize selected pathways overrepresented in the endometrium of day 7 post-ovulation induction of cows treated to ovulate larger versus smaller follicles
Methods: Seventy-four multiparous, nonlactating, presynchronized Nelore cows received a progesterone-releasing device and estradiol benzoate on Day–10 (D−10). Animals received cloprostenol (large follicle-large CL group; LF-LCL; N = 35) or not (small follicle-small CL group; SF-SCL; N = 39) on D−10. Progesterone devices were withdrawn and cloprostenol administered 42 to 54 hours (LF-LCL) or 30 to 36 hours (SF-SCL) before GnRH treatment (D0). Tissues were collected at slaughter on D7. Transcriptional profiling of the endometrial tissue was performed by RNA-seq and protein markers for proliferation and apoptosis were identified by immunohistochemistry.
Results: Functional enrichment data indicated that LF-LCL endometrium expressed greater abundance of genes associated with biosynthetic and metabolic processes, whereas SF-SCL endometrium .gene expression profile was biased towards cell proliferation. Extracellular matrix-related genes were also upregulated in the SF-SCL endometrium suggesting reorganization of the ECM towards a proliferation permissive phenotype. Immunohistochemistry data confirmed the greater proliferative activity observed in SF-SCL endometrium I comparison to LF-LCL counterparts, and indicated a time-related transition within experimental group. In conclusion, the periovulatory endocrine milieu regulates bovine endometrial gene expression. Furthermore, timing and amplitude of ovarian steroid secretion pattern seem to determine the transition from a proliferation permissive to a biosynthetic and metabolically active endometrial phenotype, which may be associated with the preparation of an optimally receptive uterine environment.
Conclusion: the periovulatory endocrine milieu affected D7 endometrial molecular signature. Main pathways affected were related to cell proliferation, ECM composition and remodeling, biosynthetic and metabolic processes. Reported data further suggest that the endometrial tissue from LF-LCL cows experienced an early proliferative phase (D4), whereas the SF-SCL endometrium, exposed to a distinct periovulatory endocrine environment, expressed a delayed onset of the proliferative activity (D7).
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| Overall design |
endometrial mRNA profiles of endocrine manipulated cows were generated by deep sequencing using Illumina HiScanSQ platform
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| Contributor(s) |
Mesquita F, Ramos R, Andrade S, Gasparin G, Gonella A, Lehmann Coutinho L, Binelli M |
| Citation(s) |
26178716, 26981354 |
| BioProject |
PRJNA270391 |
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| Submission date |
Jan 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mario Binelli |
| E-mail(s) |
binelli@usp.br
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| Phone |
19998591388
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| Organization name |
University of Sao Paulo (USP)
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| Department |
department of animal reproduction
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| Lab |
Molecular Physiology and Endocrinology
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| Street address |
Rua Duque de Caxias Norte, 255
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| City |
Pirassununga |
| State/province |
SP |
| ZIP/Postal code |
13635-900 |
| Country |
Brazil |
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| Platforms (1) |
| GPL19671 |
Illumina HiScanSQ (Bos indicus) |
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| Samples (6)
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| Relations |
| SRA |
SRP051330 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65450_Mesquita_processed_data.txt.gz |
408.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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