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| Status |
Public on Oct 13, 2016 |
| Title |
ALKBH1-Mediated Transfer RNA Demethylation |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Transfer RNA (tRNA) is a central component of protein synthesis and cell signaling network. One salient feature of tRNA is its heavily modified status, which can critically impact its function. Here we show that mammalian ALKBH1 is a tRNA demethylase. It mediates the demethylation of N1-methyladenosine (m1A) in tRNAs. The ALKBH1-catalyzed demethylation of the target tRNAs results in their reduced partition in polysomes and their decreased usage in protein synthesis. This process is dynamic and responds to glucose availability to affect translation. Our results uncover reversible methylation of tRNA as a new regulatory mechanism of post-transcriptional gene expression.
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| |
|
| Overall design |
Profile the binding RNA of ALKBH1 by CLIP-seq.
tRNA-seq wild-type and Alkbh1 KOMEF
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| |
|
| Contributor(s) |
Liu F, He C, Wei J |
| Citation(s) |
27745969 |
| |
| Submission date |
Jan 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Guan-Zheng Luo |
| Organization name |
UChicago
|
| Department |
Chemistry
|
| Street address |
929 E 57th St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (9)
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|
| Relations |
| BioProject |
PRJNA273642 |
| SRA |
SRP052831 |
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