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Series GSE65299 Query DataSets for GSE65299
Status Public on Oct 13, 2016
Title ALKBH1-Mediated Transfer RNA Demethylation
Organisms Homo sapiens; Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Transfer RNA (tRNA) is a central component of protein synthesis and cell signaling network. One salient feature of tRNA is its heavily modified status, which can critically impact its function. Here we show that mammalian ALKBH1 is a tRNA demethylase. It mediates the demethylation of N1-methyladenosine (m1A) in tRNAs. The ALKBH1-catalyzed demethylation of the target tRNAs results in their reduced partition in polysomes and their decreased usage in protein synthesis. This process is dynamic and responds to glucose availability to affect translation. Our results uncover reversible methylation of tRNA as a new regulatory mechanism of post-transcriptional gene expression.
Overall design Profile the binding RNA of ALKBH1 by CLIP-seq.
tRNA-seq wild-type and Alkbh1 KOMEF
Contributor(s) Liu F, He C, Wei J
Citation(s) 27745969
Submission date Jan 26, 2015
Last update date May 15, 2019
Contact name Guan-Zheng Luo
Organization name UChicago
Department Chemistry
Street address 929 E 57th St
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM2107198 CLIP-ALKBH1-replicate1
GSM2107199 CLIP-ALKBH1-replicate2
GSM2107200 CLIP-ALKBH1-replicate3
BioProject PRJNA273642
SRA SRP052831

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65299_RAW.tar 6.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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