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| Status |
Public on May 21, 2015 |
| Title |
Temporal Changes in Rat Liver Gene Expression after Acute Cadmium and Chromium Exposure |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
U.S. Service Members and civilians are at risk of exposure to a variety of environmental health hazards throughout their normal duty activities and in industrial occupations. Metals are widely used in large quantities in a number of industrial processes and are a common environmental toxicant, which increases the possibility of being exposed at toxic levels. While metal toxicity has been widely studied, the exact mechanisms of toxicity remain unclear. In order to further elucidate these mechanisms and identify candidate biomarkers, rats were exposed via a single intraperitoneal injection to three concentrations of CdCl2 and Na2Cr2O7, with livers harvested at 1, 3, or 7 days after exposure. Cd and Cr accumulated in the liver at 1 day post exposure. Cd levels remained elevated over the length of the experiment, while Cr levels declined. Metal exposures induced ROS, including hydroxyl radical (•OH), resulting in DNA strand breaks and lipid peroxidation. Interestingly, ROS and cellular damage appeared to increase with time post-exposure in both metals, despite declines in Cr levels. Differentially expressed genes were identified via microarray analysis. Both metals perturbed gene expression in pathways related to oxidative stress, metabolism, DNA damage, cell cycle, and inflammatory response. This work provides insight into the temporal effects and mechanistic pathways involved in acute metal intoxication, leading to the identification of candidate biomarkers.
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| Overall design |
Rats (5 per group) were exposed via a single intraperitoneal injection to three concentrations of CdCl2, Na2Cr2O7, or vehicle control,with livers harvested at 1, 3, or 7 days after exposure. Only the Cd mid dose and Cr high dose were selected for microarray analysis.
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| Contributor(s) |
Madejczyk MS, Baer CE, Dennis WE, Minarchick VC, Leonard SS, Jackson DA, Stallings JD, Lewis JA |
| Citation(s) |
25993096 |
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| Submission date |
Jan 22, 2015 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Michael Scott Madejczyk |
| Organization name |
US Army Center for Environmental Health Research
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| Department |
Environmental Health Program
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| Street address |
568 Doughten Dr
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| City |
Fort Detrick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (59)
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| GSM1589396 |
Cd Control Day 1, Biological rep 4 |
| GSM1589397 |
Cd Control Day 1, Biological rep 5 |
| GSM1589398 |
Cd 1.25 mg/kg Day 1, Biological rep 1 |
| GSM1589399 |
Cd 1.25 mg/kg Day 1, Biological rep 2 |
| GSM1589400 |
Cd 1.25 mg/kg Day 1, Biological rep 4 |
| GSM1589401 |
Cd 1.25 mg/kg Day 1, Biological rep 5 |
| GSM1589402 |
Cd Control Day 3, Biological rep 1 |
| GSM1589403 |
Cd Control Day 3, Biological rep 2 |
| GSM1589404 |
Cd Control Day 3, Biological rep 3 |
| GSM1589405 |
Cd Control Day 3, Biological rep 4 |
| GSM1589406 |
Cd Control Day 3, Biological rep 5 |
| GSM1589407 |
Cd 1.25 mg/kg Day 3, Biological rep 1 |
| GSM1589408 |
Cd 1.25 mg/kg Day 3, Biological rep 2 |
| GSM1589409 |
Cd 1.25 mg/kg Day 3, Biological rep 3 |
| GSM1589410 |
Cd 1.25 mg/kg Day 3, Biological rep 4 |
| GSM1589411 |
Cd 1.25 mg/kg Day 3, Biological rep 5 |
| GSM1589412 |
Cd Control Day 7, Biological rep 1 |
| GSM1589413 |
Cd Control Day 7, Biological rep 2 |
| GSM1589414 |
Cd Control Day 7, Biological rep 3 |
| GSM1589415 |
Cd Control Day 7, Biological rep 4 |
| GSM1589416 |
Cd Control Day 7, Biological rep 5 |
| GSM1589417 |
Cd 1.25 mg/kg Day 7, Biological rep 1 |
| GSM1589418 |
Cd 1.25 mg/kg Day 7, Biological rep 2 |
| GSM1589419 |
Cd 1.25 mg/kg Day 7, Biological rep 3 |
| GSM1589420 |
Cd 1.25 mg/kg Day 7, Biological rep 4 |
| GSM1589421 |
Cd 1.25 mg/kg Day 7, Biological rep 5 |
| GSM1589422 |
Cr Control Day 1, Biological rep 1 |
| GSM1589423 |
Cr Control Day 1, Biological rep 2 |
| GSM1589424 |
Cr Control Day 1, Biological rep 3 |
| GSM1589425 |
Cr Control Day 1, Biological rep 4 |
| GSM1589426 |
Cr Control Day 1, Biological rep 5 |
| GSM1589427 |
Cr 20 mg/kg Day 1, Biological rep 1 |
| GSM1589428 |
Cr 20 mg/kg Day 1, Biological rep 2 |
| GSM1589429 |
Cr 20 mg/kg Day 1, Biological rep 3 |
| GSM1589430 |
Cr 20 mg/kg Day 1, Biological rep 4 |
| GSM1589431 |
Cr 20 mg/kg Day 1, Biological rep 5 |
| GSM1589432 |
Cr Control Day 3, Biological rep 1 |
| GSM1589433 |
Cr Control Day 3, Biological rep 2 |
| GSM1589434 |
Cr Control Day 3, Biological rep 3 |
| GSM1589435 |
Cr Control Day 3, Biological rep 4 |
| GSM1589436 |
Cr Control Day 3, Biological rep 5 |
| GSM1589437 |
Cr 20 mg/kg Day 3, Biological rep 1 |
| GSM1589438 |
Cr 20 mg/kg Day 3, Biological rep 2 |
| GSM1589439 |
Cr 20 mg/kg Day 3, Biological rep 3 |
| GSM1589440 |
Cr 20 mg/kg Day 3, Biological rep 4 |
| GSM1589441 |
Cr 20 mg/kg Day 3, Biological rep 5 |
| GSM1589442 |
Cr Control Day 7, Biological rep 1 |
| GSM1589443 |
Cr Control Day 7, Biological rep 2 |
| GSM1589444 |
Cr Control Day 7, Biological rep 3 |
| GSM1589445 |
Cr Control Day 7, Biological rep 4 |
| GSM1589446 |
Cr Control Day 7, Biological rep 5 |
| GSM1589447 |
Cr 20 mg/kg Day 7, Biological rep 1 |
| GSM1589448 |
Cr 20 mg/kg Day 7, Biological rep 2 |
| GSM1589449 |
Cr 20 mg/kg Day 7, Biological rep 3 |
| GSM1589450 |
Cr 20 mg/kg Day 7, Biological rep 4 |
| GSM1589451 |
Cr 20 mg/kg Day 7, Biological rep 5 |
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| Relations |
| BioProject |
PRJNA273384 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65198_RAW.tar |
142.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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