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Series GSE65107 Query DataSets for GSE65107
Status Public on Jan 19, 2016
Title Diarrhea in lymphocytic colitis: ERK1/2-dependent ENaC dysregulation and claudin-4-, -5- and -8-related barrier defects
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Background: Lymphocytic colitis (LC) causes watery diarrhea. This study aimed to identify the inherent pathomechanisms of epithelial transport and barrier dysfunction and regulatory inputs.
Methods: 8 (4 LC samples and 4 unrelated control samples) biopsies of fresh sigmoid colon were analysed by paired end sequencing (Illumina High Seq 2500)
Conclusions: ENaC-mediated Na+ malabsorption via ERK1/2 and epithelial barrier dysfunction from tight junction downregulation through claudin-4, -5, and -8 are mechanisms causing malabsorptive and leak flux diarrhea in LC resulting from the key effector cytokines TNFa and INFg.
Overall design Sigmoid colon mRNA profiles from 8 unrelated individuals (4 with LC, 4 as control) were generated using paired end sequencing with Illumina High Seq 2500
Contributor(s) Barmeyer C, Erko I, Fromm A, Bojarski C, Ploeger S, Richter JF, Loddenkemper C, Dames P, Kerick M, Siegmund B, Fromm M, Schweiger MR, Schulzke J
Citation(s) 26658215
Submission date Jan 20, 2015
Last update date May 15, 2019
Contact name Michal Ruth Schweiger
Organization name Max Planck Institute For Molecular Genetics
Department Lehrach
Lab Schweiger
Street address Ihnestrasse 63-73
City Berlin
ZIP/Postal code 14195
Country Germany
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM1587421 Ctrl rep1
GSM1587422 Ctrl rep2
GSM1587423 Ctrl rep3
BioProject PRJNA273003
SRA SRP052615

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Supplementary file Size Download File type/resource
GSE65107_LymphycyticColitis_Ratios_RawCounts_CPM.txt.gz 18.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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