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| Status |
Public on Jan 16, 2015 |
| Title |
Lymph node CD4+ T cell expression data from naïve female offspring of C57BL/6J and C57BL/6J-ChrY^SJL |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The prevalence of some autoimmune diseases (AID) is greater in females compared with males, notwithstanding that disease severity is often greater in males. The reason for this sexual dimorphism (SD) is unknown, but may reflect negative selection of Y chromosome (ChrY) bearing sperm during spermatogenesis or male fetuses early in the course of conception/pregnancy. Previously, we showed that the SD in experimental autoimmune encephalomyelitis (EAE) is associated with copy number variation (CNV) in ChrY multicopy genes. Here, we test the hypothesis that CNV in ChrY multicopy genes influences the paternal parent-of-origin effect on EAE susceptibility in female mice. We show that C57BL/6J consomic strains of mice possessing an identical ChrX and CNV in ChrY multicopy genes exhibit a female biased sex-ratio and sperm head abnormalities, consistent with X-Y intragenomic conflict arising from an imbalance in CNV between homologous ChrX:ChrY multicopy genes. These males also display paternal transmission of EAE to female offspring and differential loading of miRNAs within the sperm nucleus. These findings provide evidence for a genetic mechanism at the level of the male gamete that contributes to the SD in EAE and paternal parent-of-origin effects in female mice, raising the possibility that a similar mechanism may contribute to the SD in MS.
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| Overall design |
Three biological replicates for each strain were pooled from the axillary, brachial, and inguinal lymph nodes from 5 mice for each replicate. RNA was isolated from CD4+TCRB+ FAC sorted cells.
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| Contributor(s) |
Case LK, Wall EH, Osmanski EE, Dragon JA, Saligrama N, Zachary JF, Lemos B, Blankenhorn EP, Teuscher C |
| Citation(s) |
25886764 |
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| Submission date |
Jan 15, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Laure Case |
| E-mail(s) |
lcase@uvm.edu
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| Organization name |
University of Vermont
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| Street address |
89 Beaumont Ave
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| City |
Burlington |
| ZIP/Postal code |
05405 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE65048 |
Copy number variation in Y chromosome multicopy genes is linked to a paternal parent-of-origin effect on CNS autoimmune disease in female offspring |
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| Relations |
| BioProject |
PRJNA272746 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65038_RAW.tar |
25.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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