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Series GSE64778 Query DataSets for GSE64778
Status Public on Dec 08, 2015
Title Changes in transcript expression in circulating whole blood resulting from exposure to neurotoxic doses of D-amphetamine or Heat Stroke/Hyperthermia
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary These experiments were designed to detect transcript (mRNA) changes in whole circulating blood in animals exposed to D-amphetamine under neurotoxic and non-neurotoxic conditions, or subjected to elevated environmental temperatures that produced a hyperthermia very similar to heat stroke. The study objectives were: 1) to detect transcript changes in blood due to life-threatening hyperthermia produced by elevated environmental temperatures (39°C, produces no or minimal neurotoxicity); 2) detect transcripts that could serve as biomarkers specific for neurotoxic amphetamine exposures and not seen with environmentally-induced hyperthermia; and 3) determine the transcript changes related to the immune system in circulating blood produced by either non-neurotoxic or neurotoxic amphetamine exposures.
Amphetamine effects on gene expression are dependent on body temperature and indicate that many significant changes in genes related to the immune system occur, some likely in response to damage, even when animals remain normothermic during amphetamine exposure. Also, hyperthermia alone produces many changes in immune related genes in blood
Overall design Five groups of animals were necessary to meet the study objectives. All groups were given 4 injections of either normal saline or amphetamine, and the injections were sequentially given with 2 h between each injection. Dosing started at 7:30 to 8:30 a.m. The groups are: 1) normothermic controls given normal saline in a 22.5°C environment; 2) controls given normal saline in a 16°C environment (also remained normothermic); 3) environmentally-induced hyperthermia given saline in a 39°C environment; 4) non-neurotoxic amphetamine given in a 16°C environment and 5) neurotoxic amphetamine group given amphetamine in a 22.5°C environment. Note the the saline controls (normothermic data) is contained in a separate but linked GEO file GSE62368
Contributor(s) Bowyer JF, Crabtree NM, George NI
Citation(s) 26177368
Submission date Jan 08, 2015
Last update date May 15, 2019
Contact name John Francis Bowyer
Phone 870-543-7194
Organization name National Center for Toxicological Research FDA
Department Neurotoxicology
Lab Neurobiology
Street address 3900 NCTR Drive
City Jefferson
State/province AR
ZIP/Postal code 72079-9502
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (64)
GSM1525865 EIH ID ML324
GSM1525866 EIH ID JB90
GSM1525867 EIH ID JB109
BioProject PRJNA271851
SRA SRP052323

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Supplementary file Size Download File type/resource
GSE64778_RAW.tar 11.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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