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| Status |
Public on Oct 22, 2015 |
| Title |
Differences in murine miR-150-/- CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
MicroRNAs are a major class of gene regulators in mammals. While numerous aspects of the immune systems are controlled by miRNAs, their precise role in the CD8+ T cell response remains unclear. In this report, we show that miR-150 is the most abundant miRNA expressed in CD8+ T cells and its expression is required for proper effector cell differentiation in response to acute and chronic pathogens. In the absence of miR-150, CD8+ T cells failed to both undergo robust expansion and differentiate into short-lived terminal effector cells. The lack of miR-150 also altered the effector CD8+ T cell transcriptome such that, despite activation, genes associated with naïve or memory cells were highly expressed. The deletion of miR-150 also reduced killing efficiency of CD8+ T cells. These results uncover a cell-intrinsic role for miR-150 in the regulation of CD8+ T cell effector fate and function.
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| Overall design |
mRNA and small RNAs were sequenced each from 2 biological replicates of wild-type and miR-150 KO CD8+ T cells
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| Contributor(s) |
Wissink EM, Grimson A |
| Citation(s) |
26549197 |
| |
| Submission date |
Jan 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Grimson |
| Organization name |
Cornell University
|
| Department |
Molecular Biology & Genetics
|
| Street address |
445 Biotechnology Building
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA271681 |
| SRA |
SRP051727 |
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