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Series GSE64649 Query DataSets for GSE64649
Status Public on Mar 02, 2023
Title Genome activity during the emergence of mouse embryonic totipotency (time course)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.
Overall design 2h interval time course after ICSI produced mouse single embryo: Biological replicates: 3 replicates at 0h, 3 replicates at 2h, 4 replicates at 4h, 3 replicates at 6h, 3 replicates at 8h, 4 replicates at 10h, 4 replicates at 12h
Contributor(s) Perry AC
Citation(s) 36729835
Submission date Jan 05, 2015
Last update date Mar 03, 2023
Contact name Xin Lu
Organization name University of Regensburg
Street address Universit├Ątsstra├če 31
City Regensburg
ZIP/Postal code 93053
Country Germany
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (24)
GSM1576212 mII oocyte t.0h replicate_a
GSM1576213 mII oocyte t.0h replicate_c
GSM1576214 mII oocyte t.0h replicate_d
This SubSeries is part of SuperSeries:
GSE64650 Genome activity during the emergence of mouse embryonic totipotency
BioProject PRJNA271563

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64649_RAW.tar 110.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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