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Series GSE63886 Query DataSets for GSE63886
Status Public on Apr 01, 2015
Title Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the underlying mechanism is still unclear. To address this question, we analysed gene expression profiles of SCNT-derived 2-cell mouse embryos treated with trichostatin A (TSA), a potent HDAC inhibitor that is best used for mouse cloning. Unexpectedly, TSA had no effect on the numbers of aberrantly expressed genes or the overall gene expression pattern in the embryos. However, in-depth investigation by gene ontology and functional analyses revealed that TSA treatment specifically improved the expression of a small subset of genes encoding transcription factors and their regulatory factors, suggesting their positive involvement in de novo RNA synthesis. Indeed, introduction of one of such transcription factors, Spi-C, into the embryos at least partially mimicked the TSA-induced improvement in embryonic development by activating gene networks associated with transcriptional regulation. Thus, the effects of TSA treatment on embryonic gene expression did not seem to be stochastic, but more specific than expected, targeting genes that direct development and trigger zygotic genome activation at the 2-cell stage.
 
Overall design Gene expression were measured in mouse unfertilized oocytes, in vitro fertilized, somatic cell cloned embryos and cumulus cells. Somatic cell cloned embryos were treated with/without TSA or Spi-C mRNA and subjected at 2-cell stage. More than four biological replicates were performed in each group.
 
Contributor(s) Inoue K, Oikawa M, Kamimura S, Ogonuki N, Nakamura T, Nakano T, Abe K, Ogura A
Citation(s) 25974394
Submission date Dec 05, 2014
Last update date Jan 12, 2017
Contact name Kimiko Inoue
E-mail(s) kimiko.inoue@riken.jp
Organization name BRC, RIKEN
Department Bioresource Engineering Division
Street address 3-1-1 Koyadai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-0074
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (39)
GSM1559400 unfertilized_oocyte_replication1
GSM1559401 unfertilized_oocyte_replication2
GSM1559402 unfertilized_oocyte_replication3
Relations
BioProject PRJNA269330

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63886_RAW.tar 78.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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