|
| Status |
Public on Nov 15, 2015 |
| Title |
Rb family triple knock out liver progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This serie was performed on liver progenitor isolated based on their expression of the Sca1 surface marker. It contains two independent samples from Rb family deficient liver and three independent samples from control liver, all isolated 2 weeks after Tamoxifen-induced Rb family ablation. Hematopoietic (CD45, Ter119) and endothelial (CD31) markers were used as negative selection markers to exclude blood or endothelial cells from the isolated fraction.
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| |
|
| Overall design |
Each liver was digested to obtain single cell suspension. Cells were positively enriched on column for Sca1 expression and further stained with antibodies against CD45, Ter119 and Cd31. Each sample represents 20-30K cells isolated by flow cytometry and sorted directly into Trizol
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| |
|
| Contributor(s) |
Viatour P |
| Citation(s) |
26639898 |
| |
| Submission date |
Dec 02, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Pichai Raman |
| E-mail(s) |
pichai.raman@gmail.com
|
| Phone |
(267)425-0143
|
| Organization name |
Children's Hospital of Philadelphia
|
| Street address |
3501 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (5)
|
| GSM1557524 |
Rb family triple Knock Out liver progenitor sample 1 |
| GSM1557525 |
Rb family triple Knock Out liver progenitor sample 2 |
| GSM1557526 |
Control liver progenitor sample 1 |
|
| Relations |
| BioProject |
PRJNA269076 |
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