|
| Status |
Public on Jan 16, 2008 |
| Title |
Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The 4 Fra2-targeting siRNA and 4 control-siRNA samples were used for analysis.
Keywords: siRNA
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| |
|
| Overall design |
A microarray procedure was performed according to the Affymetrix protocols (Santa Clara, CA). In brief, total RNA extracted from the tumor samples was analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and cRNA was synthesized with the GeneChip® 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). The labeled cRNAs were then purified and used for probes. Hybridization was performed on the Affymetrix GeneChip HG-U133 Plus2.0 array for 16 hours at 45 ºC. Signal intensity was measured by a GeneChip®Scanner3000 (Affymetrix) and converted to numerical data with GeneChip Operating Software, Ver.1 (Affymetrix).
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| |
|
| Contributor(s) |
Nakayama T, Nishio K, Arao T |
| Citation(s) |
18071306 |
| Submission date |
Nov 28, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Tokuzo Arao |
| E-mail(s) |
arao@med.kindai.ac.jp
|
| Organization name |
Kinki University School of Medicine
|
| Department |
Department of Genome Biology
|
| Street address |
377-2 Ohno-Higashi, Osaka-Sayama
|
| City |
Osaka |
| ZIP/Postal code |
589-8511 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
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|
| Relations |
| BioProject |
PRJNA99649 |
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