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| Status |
Public on Dec 02, 2014 |
| Title |
Expression data from bone marrow neutrophils with or without LPS stimulation in wild type and miR-125a deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Neutrophil activation plays a critical role in the inflammatory response to gram-negative bacterial infections. Lipopolysaccharide (LPS) from gram-negative bacterial has been shown to be a major mediator of neutrophil activation to produce pro-inflammatory cytokines, chemokines and ROS which are important to tissue damage in LPS induced septic shock.
We used microarrays to detail the global gene expression of neutrophils from miR-125a+/+ and miR-125a-/- mice after LPS stimulation.
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| Overall design |
Bone marrow neutrophils from miR-125a deficient and wild type mice were enriched by magnetic beads, then stimulated without or with LPS for 3 hours and harvested in Trizol . Extracted RNAs from three independent experiments were pooled for hybridization on Affymetrix microarrays.
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| Contributor(s) |
Shen N, Yao Y, Qin Y, Wu L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 01, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Nan Shen |
| E-mail(s) |
nanshensibs@gmail.com
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| Organization name |
Shanghai Institute of Rheumatology
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| Street address |
145 Shan Dong Middle Road
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| City |
shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA268949 |
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