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Series GSE63576 Query DataSets for GSE63576
Status Public on Dec 10, 2015
Title Somatosensory Neuron Types Identified by High-Coverage Single-Cell RNA-Sequencing and Functional Heterogeneity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 ± 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
Overall design RNA-seq of mRNA levels in 197 individual DRG neurons
We performed RNA-seq on total 203 individual DRG neurons. Six of them were not qualified and thus, were excluded for further analysis. To evaluate the quality of RNA-seq, we randomly devided No.72 neurons into two parts and performed RNA-seq seperately. Thus, we had 204 individual samples from 203 individual DRG and 198 individual qualified samples from 197 individual DRG. To evaluate the homogeneity of RNA-seq data from different mice at the same age just as used, we performed RNA-seq on 5 single DRG from different mice. Here, these data from DRG were also considered as experimental control.
The 'DRG_neurons_RNA_Seq.txt' contains processed data for 204 samples and 'DRG_RNA_Seq.txt' for 5 samples.
Contributor(s) Li C, Zhang X
Citation(s) 26691752
Submission date Nov 24, 2014
Last update date May 15, 2019
Contact name Xu Zhang
Organization name Institute of neuroscience
Department Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Lab Lab of sensory system
Street address 320 Yue Yang Road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (209)
GSM1552873 2
GSM1552874 4
GSM1552875 5
BioProject PRJNA268295
SRA SRP050181

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Supplementary file Size Download File type/resource
GSE63576_DRG_RNA_Seq.txt.gz 385.2 Kb (ftp)(http) TXT
GSE63576_DRG_neurons_RNA_Seq.txt.gz 8.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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