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| Status |
Public on May 28, 2015 |
| Title |
RNA-Seq of cKIT+ sorted cells from 53-137 day old fetal testes and ovaries and RNA-Seq of TRA-1-81+ H1 and UCLA1 hESCs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-Seq analyses on 15 human fetal samples at 53-137 days of development, 9 female and 5 male, and identified the transcriptional changes during the transition of human cKIT+ primordial germ cells (PGCs), the precursors of gametes, to the generation of Advanced Germline Cells. Comparing the transcriptional profile of PGCs to that of H1 and UCLA1 hESCs identifies differences between the two cell types and pinpoints molecules that can be used in the development of in vitro germ cell differentiation protocols starting from human pluripotent stem cells.
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| Overall design |
RNA-Seq of cKIT+ cells analyzed from 6 biological samples for testes and 9 samples for ovaries from 53-137 days. 2 biological replicates of TRA-1-81+ cells sorted from H1 and UCLA1 hESCs. WGBS of cKIT+ cells analyzed from 4 biological samples of ovaries and 1 biological sample of testes at 57-137 days of development.
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| Contributor(s) |
Gkountela S, Zhang KX, Shafiq TA, Hargan-Calvopiña J, Chen P, Clark AT |
| Citation(s) |
26004067 |
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| Submission date |
Nov 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Sofia Gkountela |
| E-mail(s) |
sgkountela@ucla.edu
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| Organization name |
UCLA
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| Department |
MCDB
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| Lab |
Amander Clark
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| Street address |
621 Charles E Young Dr S
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (19)
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| GSM1547999 |
UW203, cKIT+ RNA Seq Ovaries |
| GSM1548000 |
UW168, cKIT+ RNA Seq Ovaries |
| GSM1548001 |
UW169, cKIT+ RNA Seq Ovaries |
| GSM1548002 |
UW180, cKIT+ RNA Seq Ovaries |
| GSM1548003 |
UW202, cKIT+ RNA Seq Ovaries |
| GSM1548004 |
UW162, cKIT+ RNA Seq Ovaries |
| GSM1548005 |
UW93, cKIT+ RNA Seq Testes |
| GSM1548006 |
UW157, cKIT+ RNA Seq Testes |
| GSM1548007 |
UW161, cKIT+ RNA Seq Testes |
| GSM1548008 |
UW177cKIT+ RNA Seq Testes |
| GSM1548009 |
UW170, cKIT+ RNA Seq Testes |
| GSM1548010 |
UW165, cKIT+ RNA Seq Testes |
| GSM1548011 |
H1 TRA-1-81+ RNA Seq RepA |
| GSM1548012 |
H1 TRA-1-81+ RNA Seq RepB |
| GSM1548013 |
UCLA1 TRA-1-81+ RNA Seq RepA |
| GSM1548014 |
UCLA1 TRA-1-81+ RNA Seq RepB |
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| This SubSeries is part of SuperSeries: |
| GSE63394 |
cKIT+ sorted cells from 57-137 day old fetal testes and ovaries |
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| Relations |
| SRA |
SRP049981 |
| BioProject |
PRJNA269043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63392_all_RPKMs.txt.gz |
4.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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