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| Status |
Public on Dec 25, 2006 |
| Title |
Gene expression analysis of Peripheral T-cell Lymphoma/Unspecified |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Peripheral T-cell lymphoma unspecified (PTCL/U), the most common form of PTCL, displays heterogeneous morphology and phenotype, poor response to treatment, and dismal prognosis. We demonstrate that PTCL/U shows a gene expression profile clearly distinct from that of normal T-cells. Comparison with the profiles of purified T-cell subpopulations [CD4+, CD8+, resting (HLA-DR-), and activated (HLA-DR+)] reveals that PTCLs/U are most closely related to activated peripheral T-lymphocytes, either CD4+ or CD8+. Interestingly, the global gene expression profile cannot be surrogated by routine CD4/CD8 immunohistochemistry. When compared with normal T-cells, PTCLs/U display deregulation of functional programs often involved in tumorigenesis (e.g. apoptosis, proliferation, cell adhesion, and matrix remodeling). Products of deregulated genes can be detected in PTCLs/U by immunohistochemistry with an ectopic, paraphysiologic or stromal location. Among others, PTCLs/U aberrantly express PDGFRA, a tyrosine-kinase receptor, whose deregulation is often related to a malignant phenotype. Notably, both phosphorylation of PDGFRA and sensitivity of cultured PTCL cells to imatinib (as well as to an inhibitor of histone-deacetylase) are found. These results, which might be extended to other rarer PTCL categories, are provided with implications for tumor pathogenesis and clinical management.
Keywords: Molecular pathogenesis, molecular classification
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| Overall design |
40 cases of Peripheral T-cell lymphoma (PTCL) were analyzed, including 28 PTCL/unspecified, 6 angioimmunoblastic (AITL) and 6 anaplastic large cell lymphoma cases (ALCL). Frozen lymph-nodes collected at diagnosis (before therapy) were used. In addition, 20 samples of normal T-cells (5 CD4+, 5 CD8+, 5 HLA-DR+, and 5 HLA-DR-) collected from peripheral blood and tonsils of healthy donors were studied. The HG U133 2.0 plus microarray (Affymetrix) was adopted.
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| Contributor(s) |
Piccaluga P, Pileri SA, Dalla-Favera R |
| Citation(s) |
17304354, 25873174 |
| Submission date |
Nov 21, 2006 |
| Last update date |
May 22, 2019 |
| Contact name |
pier paolo piccaluga |
| E-mail(s) |
ppicca@med.unibo.it
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| Organization name |
university of bologna
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| Street address |
via massarenti 9
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| City |
bologna |
| ZIP/Postal code |
40138 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA99639 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6338_RAW.tar |
463.2 Mb |
(http)(custom) |
TAR (of CEL) |
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