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Series GSE63181 Query DataSets for GSE63181
Status Public on Nov 12, 2016
Title DNA methylation status of Individual 4T1 Clonal Populations
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The methylation profiles of 6 cell lines derived from single clones of the 4T1 cell lines were assessed based on genomewide methylation levels The the promoters of genes that were overexpressed in intravasating clones were found had lower methylation levels for those clones relative to the others.
Overall design Clone_Methylation
1) 23 clonal lines were established using single cell FACs sorting from the 4T1 mammary cancer cell line.
2) After establishing the lines the clones were assesed (in a pooled setting) for their capacity to intravasate the vascular system.
3) DNA methylation profilling was carried out on a genomewide scale on two intravasting clones and four non-intravasating clones.
4) The methylation levels of the gene promoters and hypomethylated regions of the intravasating clones were then compared with the same regions of non-intravasating clones.
Contributor(s) Knott SR
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Submission date Nov 11, 2014
Last update date May 15, 2019
Contact name Simon Robert Vincent Knott
Organization name Cold Spring Harbor Laboratory
Department Biology
Lab Hannon
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (14)
GSM1543529 Meth_Clone_4T1-E_Round_1
GSM1543530 Meth_Clone_4T1-E_Round_2
GSM1543531 Meth_Clone_4T1-I_Round_1
BioProject PRJNA266898
SRA SRP049697

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63181_HMR_Methylation.txt.gz 2.4 Mb (ftp)(http) TXT
GSE63181_Promoter_Methylation.txt.gz 536.1 Kb (ftp)(http) TXT
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