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| Status |
Public on Nov 12, 2016 |
| Title |
DNA methylation status of Individual 4T1 Clonal Populations |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The methylation profiles of 6 cell lines derived from single clones of the 4T1 cell lines were assessed based on genomewide methylation levels The the promoters of genes that were overexpressed in intravasating clones were found had lower methylation levels for those clones relative to the others.
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| Overall design |
Clone_Methylation
1) 23 clonal lines were established using single cell FACs sorting from the 4T1 mammary cancer cell line.
2) After establishing the lines the clones were assesed (in a pooled setting) for their capacity to intravasate the vascular system.
3) DNA methylation profilling was carried out on a genomewide scale on two intravasting clones and four non-intravasating clones.
4) The methylation levels of the gene promoters and hypomethylated regions of the intravasating clones were then compared with the same regions of non-intravasating clones.
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| Contributor(s) |
Knott SR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Simon Robert Vincent Knott |
| Organization name |
Cold Spring Harbor Laboratory
|
| Department |
Biology
|
| Lab |
Hannon
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA266898 |
| SRA |
SRP049697 |
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