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Series GSE62927 Query DataSets for GSE62927
Status Public on Jan 31, 2015
Title Doxorubicin induces double-strand breaks at CpG island promoters
Organisms Saccharomyces cerevisiae; Mus musculus
Experiment type Other
Summary Doxorubicin is a widely used chemotherapeutic drug that intercalates between DNA base-pairs and posions Topoisomerase II, although the mechanistic basis for cell killing remains speculative. Here we show that both anthracyclines and Topoisomerase II poison cause enhanced DNA double-strand breaks around CpG island promoters of active genes genome-wide. We propose that torsion-based enhancement of nucleosome turnover exposes promoter DNA, ultimately causing DNA breaks around promoters that contributes to cell killing.
Overall design We have analyzed mouse squamous cell carcinoma cells treated with doxorubicin, aclarubicin and etoposide. The direct in situ Breaks Labeling, Enrichment on Streptavidin (BLESS, PMID 23503052) method was used for mapping DNA double-strand breaks genome-wide.
Contributor(s) Yang F, Kemp CJ, Henikoff S
Citation(s) 25705119
Submission date Nov 03, 2014
Last update date May 15, 2019
Contact name Jorja Henikoff
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19379 Illumina HiSeq 2500 (Mus musculus; Saccharomyces cerevisiae)
Samples (11)
GSM1536395 SCC_Control_(20140224_15)
GSM1536396 SCC_Doxo_Low_(20140224_16)
GSM1536397 SCC_Doxo_High_(20140224_18)
BioProject PRJNA266225
SRA SRP049468

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Supplementary file Size Download File type/resource
GSE62927_RAW.tar 509.2 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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