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| Status |
Public on Nov 18, 2006 |
| Title |
Transcriptional comparison between whole kidneys from E14.5 Wnt4 mutants and wildtype mice (MG_U74Av2 platform). (GUDMAP Series ID: 7) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify a population of genes that are enriched in the renal vesicle (RV) and its derivatives using Wnt4 mutants.
Keywords: mutant comparison
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| Overall design |
Wnt4 is required for renal vesicle (RV) induction. Therefore, RV and the derivatives (s-shaped body and eventually the mature nephron) are missing in Wnt4 mutants. At E14.5, these structures are present in wildtype kidneys. Transcriptional profile comparison between E14.5 wildtype and Wnt4 mutants therefore identify genes expressed in the RV and derivatives. Minimally pooled kidney samples were used as single biological replicates. Total RNA was isolated from these pools and subjected to a single round of amplification for use on Affymetrix arrays.
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| Contributor(s) |
McMahon AP, Valerius MT |
| Citation(s) |
18346943 |
| |
| Submission date |
Nov 15, 2006 |
| Last update date |
Feb 18, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
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| Phone |
+44 131 651 8500
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| Organization name |
IGMM MRC Human Genetics Unit
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| Lab |
GUDMAP Database Group
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| Street address |
Crewe Road
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| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA100595 |
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