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Series GSE62844 Query DataSets for GSE62844
Status Public on Dec 11, 2014
Title Rfx6 target genes in the Min6b1 cell line, a pancreatic beta cell model
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Increasing evidence suggests that loss of beta cell characteristics may cause insulin secretory deficiency in diabetes but the underlying mechanisms remain unclear. Here we show that Rfx6, whose mutation leads to neonatal diabetes in man, is essential to maintain key features of functionally mature beta cells in mice. Rfx6 loss in adult beta cells leads to glucose intolerance, impaired beta cell glucose sensing and to defective insulin secretion. This is associated with reduced expression of core components of the insulin secretion pathway including GLucokinase, the Abcc8/SUR1 subunit of KATP channels and voltage-gated Ca2 channels, which are direct targets of Rfx6. Moreover, Rfx6 contributes to the silencing of the vast majority of “disallowed” genes, a group usually specifically repressed in adult beta cells, and thus to the maintenance of beta cell maturity. These findings raise the possibility that changes in Rfx6 expression or activity may contribute to beta cell failure in man.
Overall design Two ChIP-Seq experiments have been performed : 1) anti-HA ChIP-Seq on 3HA-Rfx6 transfected Min6b1 cells and 2) anti-HA ChIP-Seq on Min6b1 cells
Contributor(s) Strasser P, Gradwohl G
Citation(s) 25497096
Submission date Oct 30, 2014
Last update date May 15, 2019
Contact name Tao YE
Organization name IGBMC (CNRS/INSERM/UDS)
Street address 1 rue Laurent Fries
City Illkirch
ZIP/Postal code 67404
Country France
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM1534425 HA-Rfx6_ChIP-Seq
GSM1534426 HA-Ctrl_ChIP-Seq
BioProject PRJNA265822
SRA SRP049404

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62844_RAW.tar 290.8 Mb (http)(custom) TAR (of TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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