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Series GSE62795 Query DataSets for GSE62795
Status Public on Oct 01, 2015
Title Monocytic myeloid-derived suppressor cells accumulate in the lungs of influenza A virus-infected mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Influenza A virus (IAV) infection causes an acute respiratory disease characterized by a strong inflammatory immune response and severe immunopathology. Pro-inflammatory mechanisms are well described in the murine IAV infection model, but less is known about the mechanisms leading to the resolution of inflammation. Here, we analyzed the contribution of monocytic myeloid-derived suppressor cells (mono-MDSC) to this process. An accumulation of mono-MDSC within the lungs was observed during the course of IAV infection and phenotypic characterization of these mono-MDSC by flow cytometry and RNA-Seq revealed an activated phenotype showing both pro- and anti-inflammatory features, including the expression of iNOS by a fraction of cells in an IFN-g-dependent manner. Mono-MDSC isolated from lungs of IAV-infected animals displayed suppressive activity when tested in vitro, and iNOS inhibitors could abrogate this suppressive activity. Collectively, our data suggest that during IAV infection monocytes can differentiate into mono-MDSC, which might contribute to the prevention of immunopathology during this life-threatening disease.
Overall design Analysis of changes in the expression of mRNAs in three different groups
Contributor(s) Milanez-Almeida P, Ulas T, Glage S, Schughart K, Lutz MB, Schultze JL, Huehn J
Citation(s) 26716013
Submission date Oct 29, 2014
Last update date May 15, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM1533480 infected lung 1
GSM1533481 infected lung 4
GSM1533482 infected lung 7
BioProject PRJNA265164
SRA SRP049366

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Supplementary file Size Download File type/resource
GSE62795_processed_data.txt.gz 355.1 Kb (ftp)(http) TXT
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