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Series GSE62618 Query DataSets for GSE62618
Status Public on Apr 23, 2015
Title Methylation data from presentation and relapsed medulloblastoma tumour samples
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary We undertook a comprehensive clinical and biological investigation of serial medulloblastoma biopsies obtained at diagnosis and relapse. Combined MYC gene family amplifications and P53 pathway defects commonly emerged at relapse, and all patients in this molecular group died of rapidly progressive disease post-relapse. To study this genetic interaction, we investigated a transgenic model of MYCN-driven medulloblastoma and found spontaneous development of Trp53 inactivating mutations. Abrogation of Trp53 function in this model produced aggressive tumors that mimicked the characteristics of relapsed human tumors with combined P53-MYC dysfunction. Restoration of p53 activity, genetic and therapeutic suppression of MYCN all reduced tumor growth and prolonged survival. Our findings identify P53–MYC interactions which emerge at medulloblastoma relapse as biomarkers of clinically aggressive disease that may be targeted therapeutically. Using this dataset, assignation of medulloblastoma molecular subgroup by Illumina 450k microarray was performed for diagnostic and relapsed medulloblastoma samples to compare subgroup membership at diagnosis and relapse.
Overall design We investigated the DNA methylation profiles of 18 diagnostic and 22 relapsing samples (including 15 diagnostic / relapse pairs) using the Illumina 450k methylation microarray
Contributor(s) Schwalbe EC, Hill RM, Clifford SC
Citation(s) 25533335
Submission date Oct 22, 2014
Last update date Mar 22, 2019
Contact name Ed Schwalbe
Organization name Northumbria University
Department Applied Sciences
Street address Ellison Place
City Newcastle upon Tyne
ZIP/Postal code NE1 8ST
Country United Kingdom
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (40)
GSM1538136 1D
GSM1538137 1R
GSM1538138 2D
This SubSeries is part of SuperSeries:
GSE62626 Combined MYC and TP53 defects emerge at medulloblastoma relapse and define rapidly progressive, therapeutically targetable disease
BioProject PRJNA266444

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62618_RAW.tar 183.1 Mb (http)(custom) TAR
GSE62618_matrixIntensitiesFinal.csv.gz 149.1 Mb (ftp)(http) CSV
GSE62618_matrixProcessed.csv.gz 128.6 Mb (ftp)(http) CSV
Processed data included within Sample table
Processed data are available on Series record

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