GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE62556 Query DataSets for GSE62556
Status Public on Nov 01, 2015
Title The evolution of genic piRNA cluster loci expression patterns
Organisms Drosophila erecta; Drosophila melanogaster; Drosophila virilis; Drosophila yakuba; Oryctolagus cuniculus; Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary This study examines the conservation of Piwi-interacting RNA (piRNA) clusters that come from protein coding gene transcripts. By sequencing small RNA libraries from gonad tissues of Drosophilids and Glires we discover a diverse set of genic piRNA clusters conserved across animals. This dataset reveals new expression patterns for genic piRNA clusters and examines whether changing piRNA expression patterns correlates with sequence changes in piRNA cluster genomic sequence across a variety of animal species.
Overall design We dissected gonad tissues consisting of ovaries from wildtype adult Drosophilids (D.melanogaster, D.erecta, D.yakuba, D.virilis) and testes from wildtype pre-pubsecent and adult mouse and rats. Adult testes from rabbits were purchased from Pel-Freez Biologicals. Total or immunopreciptated RNAs were extracted from the pulvirized gonad tissues of Drosophilids and Glires. Small RNAs were purified from these samples, converted into cDNA libraries, and sequenced on an Illumina HiSeq2000.
Contributor(s) Lau NC, Chirn G
Citation(s) 26588211
Submission date Oct 21, 2014
Last update date May 15, 2019
Contact name NELSON LAU
Organization name Boston University School of Medicine
Department Biochemistry
Street address 72 E Concord St K219, Dept of Biochemistry
State/province MA
ZIP/Postal code 02118
Country USA
Platforms (7)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL13307 Illumina HiSeq 2000 (Drosophila yakuba)
Samples (25)
GSM1528798 Dmel_FC_sRNA
GSM1528799 Dmel_FC_TJ1_mutant_sRNA
GSM1528800 Dmel_FC_TJ-2_mutant_sRNA
BioProject PRJNA264407
SRA SRP049103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62556_RAW.tar 428.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap