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| Status |
Public on Mar 30, 2015 |
| Title |
Flow-induced Protein Kinase A / CREB pathway acts via BMP signaling to promote AGM hematopoiesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
By chemical modulation of the PKA/CREB and BMP pathways in isolated AGM VE-cadherin+ cells from mid-gestation embryos, we demonstrate that PKA/CREB regulates hematopoietic engraftment and clonogenicity of hematopoietic progenitors and is dependent on secreted BMP ligands through the type I BMP receptor.
We used microarray to document upregulation of PKA/CREB-BMP pathway as well as global BMP target upregulation upon PKA/CREB activation.
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| Overall design |
Isolated VE+ cells from E11.5 AGM were treated with BMP4 (4ng/ml), forskolin (25uM) or both for 8 hours before RNA isolation.
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| Contributor(s) |
Kim PG, Daley GQ |
| Citation(s) |
25870201 |
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| Submission date |
Oct 20, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Peter Geon Kim |
| Organization name |
Dana Farber Cancer Institute
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| Department |
Hematology/Oncology
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| Lab |
Ben Ebert
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| Street address |
360 Longwood Ave, Lc8202
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA264319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62517_RAW.tar |
31.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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