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Series GSE62479 Query DataSets for GSE62479
Status Public on Nov 30, 2014
Title Prevention and cure of rotavirus infection via TLR5/NLRC4-mediated production of IL-22 and IL-18
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Activators of innate immunity may have potential to combat a broad range of infectious agents. We report that treatment with bacterial flagellin prevented rotavirus (RV) infection in mice and cured chronically RV-infected mice. Protection was independent of adaptive immunity and interferon (IFN, type I and II) and required flagellin receptors Toll-like receptor 5 (TLR5) and NOD-like receptor C4 (NLRC4). Flagellin-induced activation of TLR5 on dendritic cells elicited production of the cytokine interleukin (IL)-22, which induced a protective gene expression program in intestinal epithelial cells. Flagellin also induced NLRC4-dependent production of IL-18 and immediate elimination of RV-infected cells. Administration of IL-22 and IL-18 to mice fully recapitulated the capacity of flagellin to prevent or eliminate RV infection, and thus holds promise as a broad-spectrum antiviral agent.
Overall design Total mRNA from intestinal epithelial cells of Rag1-/- mice treated with PBS, IL-18, IL-22 or IL-22/IL-18 was assayed for RNA sequencing.
Contributor(s) Chassaing B, Zhang B, Gewirtz AT
Citation(s) 25395539
Submission date Oct 18, 2014
Last update date May 15, 2019
Contact name Benoit Chassaing
Phone 404 413 3589
Organization name Georgia State University
Department Biology
Lab Gewirtz's lab
Street address 100 Piedmont Av SE
City Atlanta
State/province GA
ZIP/Postal code 30303
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM1527640 CTRL_1
GSM1527641 CTRL_2
GSM1527642 CTRL_3
BioProject PRJNA264224
SRA SRP049019

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Supplementary file Size Download File type/resource
GSE62479_RAW.tar 9.5 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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