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| Status |
Public on Dec 08, 2015 |
| Title |
Transcript expression in circulating whole blood after saline injections |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
These experiments were designed to produce the appropriate controls so that their cohort's transcript (mRNA) changes in whole circulating blood could be determined. The cohorts were exposed to D-amphetamine under a neurotoxic and non-neurotoxic condition or subjected to elevated environmental temperatures that produced a hyperthermia very similar to heat stroke. The cohort data is in a separate, but linked, GEO file.
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| Overall design |
Animals were given four doses of 1 ml/ kg of normal saline with two hours in between each dose. The animals body temperature was determined every 1 h and the rats were sacrificed 3 h after the last saline injection. Animals were not given access to food or water during this period so that they were more suitable controls for the amphetamine treatment groups
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| Contributor(s) |
Bowyer JF, Crabtree NM, George NI |
| Citation(s) |
26177368 |
| |
| Submission date |
Oct 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
John Francis Bowyer |
| E-mail(s) |
john.bowyer@fda.hhs.gov
|
| Phone |
870-543-7194
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| Organization name |
National Center for Toxicological Research FDA
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| Department |
Neurotoxicology
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| Lab |
Neurobiology
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| Street address |
3900 NCTR Drive
|
| City |
Jefferson |
| State/province |
AR |
| ZIP/Postal code |
72079-9502 |
| Country |
USA |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA264000 |
| SRA |
SRP048964 |
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