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Series GSE62283 Query DataSets for GSE62283
Status Public on Sep 30, 2015
Title Diagnosis of Early-Stage Parkinson's Disease Using Autoantibodies as Blood-based Biomarkers
Organism Homo sapiens
Experiment type Protein profiling by protein array
Summary Human serum samples from early-stage Parkinson's disease and non-diseased controls were probed onto human protein microarrays in order to identify differentially expressed autoantibody biomarkers that could be used as diagnostic indicators. Other neurodegenerative and non-neurodegenerative diseases were also used to help measure the specificity of the selected biomarkers.
 
Overall design In the study presented here, 103 Early-stage Parkinson's disease and 156 control human serum samples were probed onto human protein microarrays in order to identify differentially expressed autoantibodies. Microarray data was analyzed using several statistical significance algorithms, and autoantibodies that demonstrated significant differences in group prevelance were selected as potential biomarkers of disease. Prediction classification analysis using Invitrogen's Prospector v5.2.1 and Random Forest tested the diagnostic efficacy of the identified biomarkers. Differentiation of early-stage PD samples from other neurodegenerative and non-neurodegenerative controls (Alzheimer's disease, multiple sclerosis, and breast cancer) assessed the specificity of the selected biomarkers, while comparison with mild-moderate Parkinson's disease samples assessed their utility for use in disease staging.
 
Contributor(s) Nagele RG, DeMarshall C, Han M, Nagele EP, Sarkar A, Acharya NK, Godsey G, Goldwaser EL, Kosciuk M, Thayasivam U, Belinka B
Citation(s) 26386375
Submission date Oct 11, 2014
Last update date Oct 02, 2015
Contact name Robert G Nagele
E-mail(s) nagelero@rowan.edu
Phone 856-566-6083
Organization name UMDNJ-SOM
Department NJISA
Lab Nagele
Street address 2 Medical Center Drive
City Stratford
State/province NJ
ZIP/Postal code 08084
Country USA
 
Platforms (1)
GPL13669 Invitrogen ProtoArray v5.0
Samples (398)
GSM1524402 Alzheimer's Disease serum sample 1 AD1
GSM1524403 Alzheimer's Disease serum sample 2 AD2
GSM1524404 Alzheimer's Disease serum sample 3 AD3
Relations
BioProject PRJNA263674

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62283_Matrix_Addendum_ESPD_vs_All_Controls.xlsx 36.2 Mb (ftp)(http) XLSX
GSE62283_Matrix_Tables.txt.gz 77.0 Mb (ftp)(http) TXT
GSE62283_RAW.tar 1.0 Gb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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