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Series GSE62151 Query DataSets for GSE62151
Status Public on Jun 23, 2015
Title Upstream Anti-sense Promoters Act as Local Enhancers of Mammalian Protein-coding Genes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the function or regulation of these anti-sense promoters or the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites (TSSs) near mouse mRNAs. We find that coupled sense and anti-sense TSSs form the boundaries of an evolutionarily conserved and nucleosome-depleted regulatory region with dramatically enriched transcription factor (TF) occupancy. Notably, as the distance between sense and anti-sense TSSs increases, so does the level of TF binding and signal-dependent gene activation. We further discover a cluster of anti-sense TSSs in macrophages with an enhancer-like chromatin signature. Remarkably, this signature identifies promoters that are selectively and rapidly activated during immune challenge. We conclude that anti-sense TSSs can serve as potent, local enhancers of sense-strand gene expression by facilitating TF binding and deposition of activating histone modifications.
Overall design ChIP-seq, Start-RNA-seq, and MNase-seq from mouse bone marrow-derived macrophages (BMDMs), and Start-RNA-seq from mouse embryonic fibroblasts (MEFs) are included.
For ChIP-seq, two biological replicates are included for each of three treatment conditions; untreated, 30 minutes lipopolysaccharide (LPS), and 2 hours LPS. Each biological replicate for all ChIP conditions was sequenced in two lanes for depth, creating two technical replicates for each biological replicate. Raw files for paired technical replicates are labeled as repX.1 and repX.2, where repX.1 is technical replicate 1 and repX.2 is technical replicate 2.
For Start-RNA-seq in BMDMs, two biological replicates are included for each of three treatment conditions; untreated, 30 min LPS, and 2 hr LPS.
For MNase-seq, data from three biological replicates of untreated BMDMs are included. Four technical replicates of biological replicate 1, two technical replicates of biological replicate 2, and four technical replicates of biological replicate 3 are included. Raw files for paired technical replicates are labeled as repX.1, repX.2, repX.3, etc.
For Start-RNA-seq in untreated MEFs, two biological replicates are included. Two technical replicates for each of the MEF Start-RNA-seq biological replicates are included. Paired technical replicates are labeled as repX.1 and repX.2.
Contributor(s) Scruggs BS, Gilchrist DA, Nechaev S, Muse G, Burkholder A, Fargo DC, Adelman K
Citation(s) 26028540
Submission date Oct 07, 2014
Last update date May 15, 2019
Contact name Karen Adelman
Organization name Harvard Medical School
Department Biological Chemistry and Molecular Pharmacology
Street address 45 Shattuck St. LHRRB-201a
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (17)
GSM1520416 Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_untreated_rep1
GSM1520417 Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_untreated_rep2
GSM1520418 Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_30min_LPS_rep1
BioProject PRJNA263290
SRA SRP048719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62151_Adelman_Bl6_MEF_startRNA-seq_5pr_rep1and2_forward.bedgraph.gz 4.9 Mb (ftp)(http) BEDGRAPH
GSE62151_Adelman_Bl6_MEF_startRNA-seq_5pr_rep1and2_reverse.bedgraph.gz 4.8 Mb (ftp)(http) BEDGRAPH 303.5 Mb (ftp)(http) BW
GSE62151_Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_2hr_LPS_rep1and2_binned_normalized.wig.gz 41.0 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_30min_LPS_rep1and2_binned_normalized.wig.gz 30.2 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_RNAPolII_ChIP-seq_untreated_rep1and2_binned_normalized.wig.gz 35.3 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_2hr_LPS_rep1and2_forward_normalized.wig.gz 5.1 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_2hr_LPS_rep1and2_reverse_normalized.wig.gz 5.1 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_30min_LPS_rep1and2_forward_normalized.wig.gz 5.4 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_30min_LPS_rep1and2_reverse_normalized.wig.gz 5.4 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_untreated_rep1and2_forward_normalized.wig.gz 4.9 Mb (ftp)(http) WIG
GSE62151_Adelman_Bl6_macrophage_startRNA-seq_5pr_untreated_rep1and2_reverse_normalized.wig.gz 4.9 Mb (ftp)(http) WIG
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