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| Status |
Public on Oct 03, 2014 |
| Title |
Evolution of Context Dependent Regulation by Expansion of Feast/Famine Regulatory Proteins [ChIP-chip] |
| Organism |
Halobacterium salinarum NRC-1 |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Chromatin immunoprecipitation and microarray hybridization (ChIP-chip) experiments were carried out for all 8 FFRPs in H. salinarum NRC-1.
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| Overall design |
To study the FFRP localization in both nutrient-replete and -deplete conditions, all strains were grown in CDM and genomic DNA of samples was harvested in both early log phase and late log phase. DNA from FFRP-bound regions was collected through c-Myc-tagged protein complexes and unenriched non-IP DNA were each labeled and hybridized to the whole genome tiling array.
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| Contributor(s) |
Plaisier CL, Baliga N |
| Citation(s) |
25394904 |
| |
| Submission date |
Oct 03, 2014 |
| Last update date |
Dec 29, 2014 |
| Contact name |
Christopher L Plaisier |
| E-mail(s) |
cplaisier@systemsbiology.org
|
| Phone |
2067322139
|
| Organization name |
Institute for Systems Biology
|
| Lab |
Nitin Baliga
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13426 |
Agilent-030521 Halobacterium sp. NRC-1 Tiling V1 013324 |
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| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE62052 |
Evolution of Context Dependent Regulation by Expansion of Feast/Famine Regulatory Proteins |
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| Relations |
| BioProject |
PRJNA263042 |
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