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| Status |
Public on Nov 02, 2014 |
| Title |
m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (3p-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here we identify Mettl3, an N6-Methyladenosine (m6A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout pre-implantation epiblasts and naïve embryonic stem cells (ESCs) are depleted for m6A in mRNAs and yet, are viable. However, they fail to adequately terminate their naïve state, and subsequently undergo aberrant and restricted lineage priming at the post-implantation stage, leading to early embryonic lethality. m6A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo, and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner.
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| Overall design |
3' polyA RNA-sequencing (equivalent to Digital Gene Expression) measured in mouse Embryonic Stem Cells (ESCs) and mouse Embriod bodies (EBs) 0,4 & 8 hours after treatment with Actinomycin which halts transcription. Measured in both WT and Mettl3-KO cells.
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| Contributor(s) |
Novershtern N, Moshitch-Moshkovitz S, Geula S, Kol N, Rechavi G, Hanna J |
| Citation(s) |
25569111, 34893620 |
| |
| Submission date |
Oct 02, 2014 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Noa Novershtern |
| E-mail(s) |
noa.novershtern@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Molecular Genetics
|
| Street address |
Weizmann Institute
|
| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
| GSM1518012 |
Mettl3-KO EBs 0h after Actinomycin treatment |
| GSM1518013 |
Mettl3-KO EBs 4h after Actinomycin treatment |
| GSM1518014 |
Mettl3-KO EBs 8h after Actinomycin treatment |
| GSM1518015 |
Mettl3-KO ESCs 0h after Actinomycin treatment |
| GSM1518016 |
Mettl3-KO ESCs 4h after Actinomycin treatment |
| GSM1518017 |
Mettl3-KO ESCs 8h after Actinomycin treatment |
| GSM1518018 |
WT EBs 0h after Actinomycin treatment |
| GSM1518019 |
WT EBs 4h after Actinomycin treatment |
| GSM1518020 |
WT EBs 8h after Actinomycin treatment |
| GSM1518021 |
WT ESCs 0h after Actinomycin treatment |
| GSM1518022 |
WT ESCs 4h after Actinomycin treatment |
| GSM1518023 |
WT ESCs 8h after Actinomycin treatment |
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| This SubSeries is part of SuperSeries: |
| GSE61998 |
m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation |
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| Relations |
| BioProject |
PRJNA262905 |
| SRA |
SRP048595 |
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