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Series GSE61994 Query DataSets for GSE61994
Status Public on Nov 02, 2014
Title m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (3p-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here we identify Mettl3, an N6-Methyladenosine (m6A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout pre-implantation epiblasts and naïve embryonic stem cells (ESCs) are depleted for m6A in mRNAs and yet, are viable. However, they fail to adequately terminate their naïve state, and subsequently undergo aberrant and restricted lineage priming at the post-implantation stage, leading to early embryonic lethality. m6A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo, and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner.
Overall design 3' polyA RNA-sequencing (equivalent to Digital Gene Expression) measured in mouse Embryonic Stem Cells (ESCs) and mouse Embriod bodies (EBs) 0,4 & 8 hours after treatment with Actinomycin which halts transcription. Measured in both WT and Mettl3-KO cells.
Contributor(s) Novershtern N, Moshitch-Moshkovitz S, Geula S, Kol N, Rechavi G, Hanna J
Citation(s) 25569111, 34893620
Submission date Oct 02, 2014
Last update date Jan 10, 2022
Contact name Noa Novershtern
Organization name Weizmann Institute of Science
Department Molecular Genetics
Street address Weizmann Institute
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM1518012 Mettl3-KO EBs 0h after Actinomycin treatment
GSM1518013 Mettl3-KO EBs 4h after Actinomycin treatment
GSM1518014 Mettl3-KO EBs 8h after Actinomycin treatment
This SubSeries is part of SuperSeries:
GSE61998 m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation
BioProject PRJNA262905
SRA SRP048595

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Supplementary file Size Download File type/resource
GSE61994_DGE_normalized_levels_RefSeq.txt.gz 704.1 Kb (ftp)(http) TXT
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