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| Status |
Public on Feb 20, 2015 |
| Title |
Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [mouse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Isolation and culture of primary prostate epithelial stem/progenitor cells (PESC) has been proven difficult and ineffective. Here we present methods to grow and expand both murine and human basal PESCs long-term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-Sca1+ CD49f+Trop2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+Trop2high PESCs. The gene expression profiles of expanded basal PESCs show similarities to ES cells and Lamin B1 and PRDX1 were identified as novel PESC markers. If transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules demonstrating their stem cell activity in vivo. The novel methods will facilitate the cellular, molecular and genomic characterization of normal and pathologic prostate glands of mouse and human origin.
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| Overall design |
Murine prostate cells were seperately cultured under spheroid and adherent stem cell conditions and subsequently used for RNA extraction
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| Contributor(s) |
Eisen C, Höfner T |
| Citation(s) |
25702639 |
| |
| Submission date |
Sep 29, 2014 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Eisen Christian |
| Organization name |
German Cancer Research Center
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| State/province |
(Click to Select US State) |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (12)
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| GSM1515853 |
murine prostate stem cells adherent monolayer cells_mouse 1_replicate 1 |
| GSM1515854 |
murine prostate stem cells adherent monolayer cells_mouse 1_replicate 2 |
| GSM1515855 |
murine prostate stem cells adherent monolayer cells_mouse 1_replicate 3 |
| GSM1515856 |
murine prostate stem cells adherent monolayer cells_mouse 1_pool of replicates 1-3 |
| GSM1515857 |
murine prostate stem cells adherent monolayer cells_mouse 2_replicate 1 |
| GSM1515858 |
murine prostate stem cells adherent monolayer cells_mouse 2_replicate 2 |
| GSM1515859 |
murine prostate stem cells adherent monolayer cells_mouse 2_replicate 3 |
| GSM1515860 |
murine prostate stem cells adherent monolayer cells_mouse 2_pool of replicates 1-3 |
| GSM1515861 |
murine prostate stem cells spheroid cells_mouse 1_replicate 1 |
| GSM1515862 |
murine prostate stem cells spheroid cells_mouse 1_replicate 2 |
| GSM1515863 |
murine prostate stem cells spheroid cells_mouse 1_replicate 3 |
| GSM1515864 |
murine prostate stem cells spheroid cells_mouse 1_pool of replicates 1-3 |
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| This SubSeries is part of SuperSeries: |
| GSE61861 |
Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin |
|
| Relations |
| BioProject |
PRJNA262634 |
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