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| Status |
Public on Sep 29, 2015 |
| Title |
The Ubiquitin Ligase Siah2 Regulates Obesity-induced Adipose Tissue Inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chronic, low-grade adipose tissue inflammation associated with adipocyte hypertrophy is an important link in the relationship between obesity and insulin resistance. Although ubiquitin ligases are essential regulators of inflammatory processes, the role of these enzymes in metabolically driven adipose tissue inflammation is relatively unexplored. In this study, we found that the ubiquitin ligase Siah2 is a central factor in obesity-related adipose tissue inflammation. When challenged with chronic excess energy intake, Siah2-null mice become obese with enlarged adipocytes, but do not develop obesity-induced insulin resistance. Proinflammatory gene expression is substantially reduced in the Siah2-null epididymal adipose tissue of the obese Siah2KO mice.
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| Overall design |
Twelve samples from four groups: WT low fat diet (LFD), WT high fat diet (LFD), Siah2KO LFD, Siah2KO HFD. There are three replicates for each group. For the replicates, equal amounts of RNA from each animal was combined into 3 pools/group for the four groups. The WT LFD, WT HFD and Siah2KO LFD groups each had 10 animals that were combined into 3, 3, or 4 animals/pool. The Siah2KO HFD group had 8 animals that were combined into 3,3, and 2 animals/pool.
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| Contributor(s) |
Floyd E, Kilroy G, Carter LE, Newman SS, Ghosh S |
| Citation(s) |
26380945 |
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| Submission date |
Sep 29, 2014 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Elizabeth Floyd |
| E-mail(s) |
floydez@pbrc.edu
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| Phone |
225-763-2724
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| Organization name |
Pennington Biomedical Research Center
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| Street address |
6400 Perkins Road
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| City |
Baton Rouge |
| State/province |
Louisiana |
| ZIP/Postal code |
70808 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA262524 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61839_Non-normalized_Data_with_gene_name.txt.gz |
4.8 Mb |
(ftp)(http) |
TXT |
| GSE61839_Non-normalized_data.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
| GSE61839_Normalized_data.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| GSE61839_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
| Processed data are available on Series record |
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