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| Status |
Public on Dec 22, 2015 |
| Title |
Next generation sequencing of miRNAs in folic acid-induced kidney damage |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We have used small RNA sequencing to measure the expression of all miRNAs present in the kidneys during the development of folic acid-induced kidney injury and fibrosis. From this miRNA expression profile, we identified miRNAs associated with different stages and aspects of kidney damage.
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| Overall design |
BALB/c mice were injected with 250mg/kg of folic acid and kidneys were collected at 0, 1, 2, 3, 7, 14, and 28 days. Total RNA was prepared for sequencing using the Illumina TruSeq Small RNA Sample Preparation Kit, and analyzed on an Illumina HiSeq 2000.
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| |
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| Contributor(s) |
Pellegrini KL, Vaidya VS |
| Citation(s) |
26707937 |
| |
| Submission date |
Sep 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kathryn Pellegrini |
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Renal Division, Department of Medicine
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA260787 |
| SRA |
SRP047031 |
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