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Series GSE61300 Query DataSets for GSE61300
Status Public on Nov 17, 2014
Title High throughput quantitative whole transcriptome analysis of CC10- B4+ and Krt5-CreERT2 - labeled distal lung cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used microfluidic single cell RNA-seq on adult isolated CC10-CreERT2 (negative) integrin beta4(pos) cells lung epithelial cells in order to determine the transcriptional profile of this putative progenitor population.
Overall design CC10-CreERT2 / tdTomato (negative) integrin beta4(pos) cells were isolated by FACS, as were Krt5-CreERT2 / tdTomato (positive) cells. These cells were pooled and loaded onto the Fluidigm C1 device.
Contributor(s) Vaughan AE, Chapman HA
Citation(s) 25533958
Submission date Sep 10, 2014
Last update date May 15, 2019
Contact name Andrew Vaughan
E-mail(s) andrewva@vet.upenn.ed
Phone 2062939309
Organization name University of Pennsylvania
Street address 3800 Spruce Street
State/province United States
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM1501785 CC10- B4+ single cells and K5-Cre+ single cells
GSM1501786 K5-Cre+ single cells
BioProject PRJNA260708
SRA SRP046995

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Supplementary file Size Download File type/resource
GSE61300_RAW.tar 13.0 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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