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| Status |
Public on May 22, 2018 |
| Title |
Transcriptional profiling of human monocyte-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this study we compared the response of human monocyte-derived macrophages differentiated with either granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF) to the most common activation stimuli: LPS plus interferon-γ to induce macrophage polarization towards the M1 type or IL-4 to induce macrophage polarization towards the M2a type. Additionally, IL-10 was used to drive M-CSF-primed macrophages into the M2c state. We used the the whole-human genome microarray to determine genes that were up- or downregulated by the activation stimuli in both macrophage lineages, with focus on genes implicated in immune response.
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| Overall design |
We generated 7 different macrophage subtypes in three biological replicates: Isolated monocytes of three healthy donors (biological replicates) were differentiated with either 25ng/ml GM-CSF or 50ng/ml M-CSF for 7d. GM-CSF-differentiated macrophages were then either mock-activated with culture medium only (control; condition 1) or activated with 100 ng/ml LPS + 25ng/ml IFNγ (condition 2), or 20 ng/ml IL-4 (condition 3) for 2d (48 h). This activation regime was also used to activate M-CSF-differentiated macrophages, which were in additionally activated with 20 ng/ml IL-10 (condition 4) for 2d (48 h).
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| Contributor(s) |
Ohradanova-Repic A, Machacek C, Stockinger H |
| Citation(s) |
29780382 |
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| Submission date |
Sep 10, 2014 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Anna Ohradanova-Repic |
| E-mail(s) |
anna.repic@meduniwien.ac.at
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| Organization name |
Medical University of Vienna
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| Street address |
Lazarettgasse 19
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (21)
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| GSM1501765 |
M-CSF Mϕ, control, biological rep 1 |
| GSM1501766 |
M-CSF Mϕ, LPS+IFNγ, biological rep 1 |
| GSM1501767 |
M-CSF Mϕ, IL-4, biological rep 1 |
| GSM1501768 |
M-CSF Mϕ, IL-10, biological rep 1 |
| GSM1501769 |
GM-CSF Mϕ, control, biological rep 2 |
| GSM1501770 |
GM-CSF Mϕ, LPS+IFNγ, biological rep 2 |
| GSM1501771 |
GM-CSF Mϕ, IL-4, biological rep 2 |
| GSM1501772 |
M-CSF Mϕ, control, biological rep 2 |
| GSM1501773 |
M-CSF Mϕ, LPS+IFNγ, biological rep 2 |
| GSM1501774 |
M-CSF Mϕ, IL-4, biological rep 2 |
| GSM1501775 |
M-CSF Mϕ, IL-10, biological rep 2 |
| GSM1501776 |
GM-CSF Mϕ, control, biological rep 3 |
| GSM1501777 |
GM-CSF Mϕ, LPS+IFNγ, biological rep 3 |
| GSM1501778 |
GM-CSF Mϕ, IL-4, biological rep 3 |
| GSM1501779 |
M-CSF Mϕ, control, biological rep 3 |
| GSM1501780 |
M-CSF Mϕ, LPS+IFNγ, biological rep 3 |
| GSM1501781 |
M-CSF Mϕ, IL-4, biological rep 3 |
| GSM1501782 |
M-CSF Mϕ, IL-10, biological rep 3 |
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| Relations |
| BioProject |
PRJNA260685 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61298_RAW.tar |
41.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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