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Series GSE61294 Query DataSets for GSE61294
Status Public on Nov 13, 2014
Title b-Catenin Mediates the Development of Behavioral Resilience [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we show that β-catenin mediates pro-resilient and anxiolytic effects in mice in the nucleus accumbens (NAc), a key brain reward region, an effect that is mediated by β-catenin signaling in D2-type medium spiny neurons (MSNs) specifically. Conversely, blocking β-catenin function in NAc promotes susceptibility to chronic stress, and we show evidence of robust suppression of β-catenin transcriptional activity in the NAc both of depressed humans examined postmortem as well as of mice that display a susceptible phenotype after chronic stress, with a converse upregulation in mice that are stress resilient. Using ChIP-seq, we demonstrate a global, genome-wide enrichment of β-catenin in the NAc of resilient mice, and specifically identify Dicer1—important in small RNA (e.g., microRNA [miRNA]) biogenesis—as a critical β-catenin target gene involved in mediating a resilient phenotype. Small RNA-seq after excising β-catenin from the NAc in the context of chronic stress reveals dynamic β-catenin-dependent miRNA regulation associated with resilience.
Overall design Control: 2 samples, Resilient: 2 samples, Susceptible: 2 samples; DNA input: 1 sample.
Contributor(s) SHAO N, DIAS C, SHEN L, NESTLER E
Citation(s) 25383518
Submission date Sep 10, 2014
Last update date May 15, 2019
Contact name Li Shen
Organization name Icahn School of Medicine at Mount Sinai
Department Neuroscience
Lab Shen
Street address 1425 Madison Ave
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (7)
GSM1501696 Control_F
GSM1501697 Control_G
GSM1501698 Defeat_A
This SubSeries is part of SuperSeries:
GSE61296 b-Catenin Mediates the Development of Behavioral Resilience
BioProject PRJNA260681
SRA SRP046987

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61294_Control.bam.tdf 293.4 Mb (ftp)(http) TDF
GSE61294_Defeat.bam.tdf 293.6 Mb (ftp)(http) TDF
GSE61294_RAW.tar 1.6 Gb (http)(custom) TAR (of TDF)
GSE61294_Resilient.bam.tdf 290.2 Mb (ftp)(http) TDF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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