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Series GSE61125 Query DataSets for GSE61125
Status Public on Apr 01, 2018
Title Transcriptional and Epigenomic profile of GSK126 or dox-mediated Ezh2 inhibition in KrasG12D/+;Trp53-/-;Ezh2i-GFP-2A-rTA;Luc lung tumors in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We wanted to understand the consequences of GSK126-mediated Ezh2 inhibition in an orthotopic model of Kras-driven non-small cell lung cancer (NSCLC). We injected the NSCLC cells with above-mentioned genotype into Nude mice and treated them with GSK126 50mg/kg (daily) or vehicle. As additional control for Ezh2 specificity we treated one tumor with doxycycline that induces shRNA-mediated Ezh2 protein downregulation in those cells. Purified tumour cells were obtained by dissection and FACS sorting based of GFP expression.
This experiment contributes the genome-wide response of NSCLC cells to Ezh2 inhibition in vivo.
Overall design We generated mRNA profiles of tumor cells tail vein injected into the lungs of Nude mice by deep sequencing. After FACS purification, RNA extraction and Bioanalyzer analysis, we processed only samples with high quality cellular and RNA profiles. Overall, we compared 10-day GSK126 treated cells (n=4) and up to 30 days GSK126 treated cells (n=3) to Captisol-treated samples (vehicle, n=2), using Illumina Hiseq2000. FACS sorted cells from individual animals were obtained by GFP expression.

For H3K27ac and H2AK5ac profiling, we used KP primary tumors generated by injection of NSCLC into the tail vein of nude mice. Mice were sacrificed on the onset of shortness of breath and tissues were resuspended in ChIP lysis buffer.
Contributor(s) Gargiulo G, Serresi M, Cesaroni M, de Rink I, Company C
Citation(s) 30487290
Submission date Sep 04, 2014
Last update date Mar 25, 2019
Contact name Gaetano Gargiulo
Organization name Max Delbrüch Center
Department Molecular Oncology
Street address Robert-Rössle-Straße 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM1497046 Vehicle-30days-rep1
GSM1497047 Vehicle-30days-rep2
GSM1497048 GSK126-10days-rep1
BioProject PRJNA260292
SRA SRP046252

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61125_RAW.tar 488.6 Mb (http)(custom) TAR (of BW)
GSE61125_expression_normalized.txt.gz 1.9 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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