|
| Status |
Public on Nov 07, 2006 |
| Title |
Sense-antisense transcript comparison in mouse brain and kidney |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparison of sense (forward probes) and antisense (reverse probes on U74 v1 gene arrays) transcripts in mouse kidney and brain.
Positive calls related to antisense transcripts were compared to the cognate signals on the 430 version of mouse genome arrays to obtain genes that co expressed sense and antisense transcripts. This had to be done manually because divergent probe IDs on the two chip generations.
Keywords: Qualitative comparison of expression
|
| |
|
| Overall design |
The first Affymetrix U74 mouse gene chips contains reversely oriented probe sets. These probes will hybridize to natural antisense transcripts that overlap with the sense transcript in the cognate area. Affymetrix provided a mask to identify the reverse probes. Positive calls with these reverse probes will give an estimate of the antisense transcriptome in that particular tissue. Comparison of the reversely oriented probes with correctly annotated probes will reveal the expression ratio of sense and antisense transcripts.
|
| |
|
| Contributor(s) |
Peters H, Jackson MS, Lastowska MA, Werner A |
| Citation(s) |
17105753 |
| |
| Submission date |
Oct 23, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Andreas Werner |
| E-mail(s) |
andreas.werner@ncl.ac.uk
|
| Phone |
+44 191 222 6990
|
| Organization name |
Newcastle University
|
| Department |
Cell and Molecular Biosciences
|
| Street address |
Framlington Place
|
| City |
Newcastle |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platforms (5)
|
| GPL32 |
[MG_U74A] Affymetrix Murine Genome U74A Array |
| GPL33 |
[MG_U74B] Affymetrix Murine Genome U74B Array |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA97575 |
Less...
More...