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| Status |
Public on Aug 29, 2014 |
| Title |
Effect of tamoxifen on early gene expression in MDA-MB-231 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Tamoxifen is the treatment of choice in estrogen receptor alpha breast cancer patients. However, ~50% of ERα-positive tumors exhibit intrinsic or rapidly acquire resistance to endocrine treatment, requiring chemotherapy. Ιt has been difficult to predict de novo resistance to endocrine therapy and/or assess the likelihood of early relapse, while no concrete mechanism regulating the acquisition and the maintenance of endocrine resistance has been identified. We have performed a whole transcriptome analysis of an ER-positive (T47D) and a triple-negative (MDA-MBA-231) breast cancer cell line exposed to tamoxifen for a short time frame (hours) in order to study resistance mechanisms that are initiated early after initiation of tamoxifen treatment.
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| Overall design |
Cells after a 4h incubation with medium containing 10% charcoal stripped FBS were incubated with vehicle,E2 (10-6M) or tamoxifen (10-6M) in RPMI 1640 supplemented with 10% charcoal stripped FBS, for 3 hours. Total RNA was isolated using Nucleospin II columns (Macheray-Nagel, Dttren, Germany), according to the manufacturer’s instructions. RNA was labeled and hybridized according to the Affymetrix protocol (Affymetrix Gene-Chip Expression Analysis Technical Manual), using the HGU133A plus 2 chip, analyzing a total of 54675 transcripts. Signals were detected by an Affymetrix microarray chip reader.
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| Contributor(s) |
Notas G, Kampa M, Castanas E |
| Citation(s) |
26115764 |
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| Submission date |
Aug 28, 2014 |
| Last update date |
Jun 05, 2019 |
| Contact name |
George Notas |
| E-mail(s) |
gnotas@uoc.gr
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| Organization name |
University of Crete
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| Department |
Medicine
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| Lab |
Experimental Endocrinology
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| Street address |
PO BOX 2208
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| City |
Heraklion |
| ZIP/Postal code |
71003 |
| Country |
Greece |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE60882 |
Effect of tamoxifen on early gene expression in T47D and MDA-MB-231 cells |
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| Relations |
| BioProject |
PRJNA259737 |
