|Public on May 04, 2015
|Microarray analysis of mouse gene expression in metronomic cyclophosphamide-treated rat glioma 9L xenografts implanted in scid mice
|Expression profiling by array
|Cyclophosphamide (CPA) treatment on a six-day repeating metronomic schedule induces a dramatic, innate immune cell-dependent regression of implanted gliomas. However, little is known about the underlying mechanisms of innate immune cell mobilization and recruitment, or about the role of DNA damage and cell stress response pathways in eliciting the anti-tumor immune responses linked to tumor regression. To address these questions, we compared untreated and 6-day metronomic cyclophosphamide-treated rat 9L gliosarcoma xenografts by mouse microarray analysis to identify responsive mouse (host) cell-specific factors.
|Rat glioma 9L tumors were implanted sc in scid immunodeficient mice then treated with cyclophosphamide at 140 mg/kg every 6 days. Tumors were collected 6 days after the fourth cyclophosphamide treatment. Tumor RNA was then analyzed on two color Agilent mouse expression microarrays comparing cyclophosphamide-treated RNA to untreated control tumor RNA.
|Doloff JC, Waxman DJ
|Aug 27, 2014
|Last update date
|Nov 01, 2017
|David J. Waxman
|Department of Biology and Bioinformatics Program
|5 Cummington Mall
|Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
|9L tumor_4th metroCPA (pool JD3 and pool JD4) versus 9L tumor_control (pool JD1 and pool JD2)_Mouse microarray
|This SubSeries is part of SuperSeries:
|Gene expression in metronomic cyclophosphamide-treated glioma xenografts