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Series GSE60740 Query DataSets for GSE60740
Status Public on Jun 30, 2015
Title Expression profiles of Ewing family of tumors authenticate distinct molecular entities
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The concept of Ewing family of tumors (EFT), characterized by FET-ETS fusions, has been recently challenged by the description of Ewing-like tumors with different gene fusions. Here we investigate the similarities and differences of FET-ETS, BCOR-CCNB3, CIC-DUX4 and EWSR1-NFATc2 tumors samples with a number of other sarcomas. Unsupervised clustering of gene expression profiles fully discriminates these four molecular entities. Specific gene signatures and pathways were further validated in model cell lines. While a clear inflammatory signature characterizes EWSR1-NFATc2 tumors, BCOR-CCNB3 and CIC-DUX4 exhibit high expression of homeobox and ETS protein families, respectively. We strongly suggest that abnormalities of chromatin remodeling may gather CIC-DUX4 and BCOR-CCNB3 tumors with rhabdoid tumors and synovial sarcomas.
 
Overall design This dataset conains 14 CIC-DUX4 and 7 EWSR1-NFATc2 tumor samples as well as Human mesenchymal cell line expressing EWSR1-NFATc2 (duplicates) or mock treated (duplicates) and IB120 CIC-DUX4 cell line expressing an shRNA directed against CIC-DUX4 (4 replicates) or mock treated (duplicates).
 
Contributor(s) Watson S, Perrin V, Szuhai K, Hogendoorn PC, Delattre O, Tirode F
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Submission date Aug 25, 2014
Last update date Jul 01, 2015
Contact name Franck Tirode
Organization name Institut Curie
Department Inserm U830
Lab Génétique et biologie des tumeurs pédiatriques
Street address 26 Rue d'Ulm
City Paris
ZIP/Postal code 75005
Country France
 
Platforms (1)
GPL17811 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray HGU133Plus2_Hs_ENTREZG_v17]
Samples (31)
GSM1486533 CIC-DUX4 tumor biopsy, sample OD_089
GSM1486534 CIC-DUX4 tumor biopsy, sample EWN1069T
GSM1486535 CIC-DUX4 tumor biopsy, sample EWN1072T
Relations
BioProject PRJNA259386

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60740_RAW.tar 138.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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