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Series GSE60595 Query DataSets for GSE60595
Status Public on Jul 22, 2016
Title Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodeling of histones and genomic 5'- methylcytosine and 5'-hydroxymethylcytosine following embryo injection were distinct from remodeling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency.
 
Overall design phICSI vs ICSI
 
Contributor(s) Perry AF
Citation(s) 27623537
Submission date Aug 21, 2014
Last update date Jan 12, 2017
Contact name Xin Lu
Organization name Universität Regensburg
Street address Universitätsstr.31
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (24)
GSM1483003 phICSI.01
GSM1483004 phICSI.04
GSM1483005 phICSI.05
Relations
BioProject PRJNA258630

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60595_RAW.tar 110.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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