|
| Status |
Public on Jul 22, 2016 |
| Title |
Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodeling of histones and genomic 5'- methylcytosine and 5'-hydroxymethylcytosine following embryo injection were distinct from remodeling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency.
|
| |
|
| Overall design |
phICSI vs ICSI
|
| |
|
| Contributor(s) |
Perry AF |
| Citation(s) |
27623537 |
| |
| Submission date |
Aug 21, 2014 |
| Last update date |
Aug 29, 2024 |
| Contact name |
Xin Lu |
| E-mail(s) |
luxgmpg@gmail.com
|
| Organization name |
University of Regensburg
|
| Street address |
Universitätsstraße 31
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA258630 |
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